Microfluidics for the Rapid Detection of Escherichia Coli O157:H7 Using Antibody-coated Microspheres

Overview

Journal Bioengineered

Date 2021 Jan 21

PMID 33472509

Citations 3

Authors

Bo Song

Jiayuan Yu

Yan Sun

Qiao Wang

Shengnan Xu

Yichen Jia

Shuying Lin

Yueying Zhang

Chen Wang

Yingbo Zhang

Xiaojie Zhang

Affiliations

Soon will be listed here.

Abstract

This study developed a novel method for the rapid detection of () O157:H7 on a microfluidic platform. First, the concentration of bacteria in a sample was determined with the adenosine triphosphate (ATP) method. Then, the specific detection of was achieved in a microfluidic chip by the immune-microsphere technique. The influences of the culture time, flow rate and capture time on the detection of the target bacteria were investigated systematically. Generally, with increasing capture time, more bacteria could be captured by the microspheres, which had a positive effect on bacterial detection. Furthermore, the sensitivity and specificity of the method were also tested. The results showed that this method could specifically detect with a sensitivity as high as 49.1 cfu/μL; the consumption of bacteria was 1 μL, and the reagent was at the microliter level. The testing time can be controlled within one and a half hours, and the cost of testing was approximately RMB 10. The method described in this article is simple and accurate and has great application value in bacterial detection for medical diagnostics.

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