L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jan 20

PMID 33466890

Citations 2

Authors

Fabiana Giglio

Maria Antonietta Castiglione Morelli

Ilenia Matera

Chiara Sinisgalli

Rocco Rossano

Angela Ostuni

Affiliations

Soon will be listed here.

Abstract

L. bulbs are commonly used as food in South Italy and also in folk medicine. By evaluating in vitro antioxidant activity and biological activities of their aqueous and methanol extracts, we shed light on the potential role, including both the nutraceutical and health benefits, of this plant. Total polyphenol content (TPC) and total flavonoid content (TFC) were evaluated by the Folin-Ciocalteu method and by the aluminum chloride method, respectively. Antioxidant activity was investigated by three in vitro assays and relative antioxidant capacity index (RACI) was calculated to compare results obtained by different tests. The extracts were tested to evaluate their possible involvement in redox homeostasis, using the human hepatoma (HepG2) cell line used as model. The extracts exhibited concentration/solvent dependent radical scavenging activity, as well as dysregulation of some genes involved in redox pathways by promoting Nrf2, SOD-2, GPX1, ABCC6 and ABCG2 expression. NMR metabolomics analysis suggests that HepG2 cells treated with extracts experience changes in some metabolites involved in various metabolic pathways.

Citing Articles

The Bioactivity and Phytochemicals of (), a Plant of Multiple Pharmacological Activities.

Jaiswal V, Lee H Int J Mol Sci. 2024; 25(5).

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Faraone I, Lela L, Ponticelli M, Gorgoglione D, De Biasio F, Valentao P Pharmaceutics. 2022; 14(6).

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