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Preparation and Culture of Bone Marrow-derived Macrophages from Mice for Functional Analysis

Overview
Journal STAR Protoc
Publisher Cell Press
Specialties Biology
Biomedical Engineering
Science
Date 2021 Jan 18
PMID 33458708
Citations 99
Authors
Gotaro Toda
Toshimasa Yamauchi
Takashi Kadowaki
Kohjiro Ueki
Affiliations
Soon will be listed here.
Abstract

The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).

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