Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry

Overview

Journal J Anal Methods Chem

Publisher Wiley

Specialty Chemistry

Date 2021 Jan 18

PMID 33457039

Citations 6

Authors

Guangqiang Huang

Jie Liang

Xiaosi Chen

Jing Lin

Jinyu Wei

Dongfang Huang

Yushan Zhou

Zhengyi Sun

Lichun Zhao

Affiliations

Soon will be listed here.

Abstract

Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.

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