Identification of 5S and 45S RDNA Sites in Chrysanthemum Species by Using Oligonucleotide Fluorescence in Situ Hybridization (Oligo-FISH)

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2021 Jan 17

PMID 33454907

Citations 12

Authors

Jun He

Sisi Lin

Zhongyu Yu

Aiping Song

Zhiyong Guan

Weimin Fang

Sumei Chen

Fei Zhang

Jiafu Jiang

Fadi Chen

Haibin Wang

Affiliations

Soon will be listed here.

Abstract

Fluorescence in situ hybridization (FISH) is a conventional method used to visualize the distribution of DNA elements within a genome. To examine the relationships within the Chrysanthemum genus, ribosomal DNA (rDNA), a popular cytogenetic marker, was utilized as a probe for FISH within this genus. Based on the genome data of Chrysanthemum nankingense, C. seticuspe and its allied genera in the Compositae(Asteraceae), we explored rDNA sequences to design oligonucleotide probes and perform oligonucleotide fluorescence in situ hybridization (Oligo-FISH) in eight Chrysanthemum accessions. The results showed that the majority of 5S rDNA signals were located in subterminal chromosome regions and that the number of 5S rDNA sites might be tightly associated with ploidy. For 45S rDNA sites, the number and intensity of signals differed from those of previously investigated Chrysanthemum resources. These findings may provide an optimally reliable method of examining the chromosome composition and structural variation of Chrysanthemum and its related species and allow researchers to understand the evolutionary history and phylogenetic relationships of Chrysanthemum.

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