Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2021 Jan 16

PMID 33450927

Citations 1

Authors

Seohyun Lee

Hyuno Kim

Hideo Higuchi

Masatoshi Ishikawa

Affiliations

Soon will be listed here.

Abstract

Elucidation of cell-level transport mediated by vesicles within a living cell provides key information regarding viral infection processes and also drug delivery mechanisms. Although the single-particle tracking method has enabled clear analysis of individual vesicle trajectories, information regarding the entire cell-level intracellular transport is hardly obtainable, due to the difficulty in collecting a large dataset with current methods. In this paper, we propose a visualization method of vesicle transport using optical flow, based on geometric cell center estimation and vector analysis, for measuring the trafficking directions. As a quantitative visualization method for determining the intracellular transport status, the proposed method is expected to be universally exploited in various biomedical cell image analyses.

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