LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating MiR-499b-5p/RWDD4 Axis

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2021 Jan 15

PMID 33447057

Citations 8

Authors

Yanyan Zheng

Jing Xie

Xiaomin Xu

Xiaoguo Yang

Yi Zhou

Qiong Yao

Ye Xiong

Affiliations

Soon will be listed here.

Abstract

Background: Long noncoding RNAs (lncRNA) exert essential functions during tumorigenesis. However, how lncRNAs participate in glioma development remains poorly researched. This study aimed to determine how DDX11-AS1 affects glioma progression.

Methods: Gene expression was analyzed by qRT-PCR. Survival rate curve was plotted in 56 glioma patients. Loss-of-function assays were performed to analyze proliferation, migration, and invasion through CCK8, colony formation, and transwell assays. Luciferase assay and RNA pulldown assays were conducted to illustrate the underlying molecular mechanism.

Results: DDX11-AS1 expression was upregulated in glioma tissues and cells. DDX11-AS1 overexpression was linked with poor prognostic value. DDX11-AS1 knockdown suppressed proliferation, migration, and invasion while inducing apoptosis. DDX11-AS1 interacted with miR-499b-5p to eliminate it, leading to upregulation of RWDD4 expression. RWDD4 was upregulated in glioma while miR-499b-5p was downregulated.

Conclusion: DDX11-AS1 upregulation promotes glioma progression through acting as a competing endogenous RNA for miR-499b-5p to upregulate RWDD4.

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References

Meng X, Deng Y, Lv Z, Liu C, Guo Z, Li Y . LncRNA SNHG5 Promotes Proliferation of Glioma by Regulating miR-205-5p/ZEB2 Axis. Onco Targets Ther. 2020; 12:11487-11496. PMC: 6939796. DOI: 10.2147/OTT.S228439. View

Li Y, Zhuang W, Huang M, Li X . Long noncoding RNA DDX11-AS1 epigenetically represses LATS2 by interacting with EZH2 and DNMT1 in hepatocellular carcinoma. Biochem Biophys Res Commun. 2019; 514(4):1051-1057. DOI: 10.1016/j.bbrc.2019.05.042. View

Perry A, Wesseling P . Histologic classification of gliomas. Handb Clin Neurol. 2016; 134:71-95. DOI: 10.1016/B978-0-12-802997-8.00005-0. View

Li B, Zhao H, Song J, Wang F, Chen M . LINC00174 down-regulation decreases chemoresistance to temozolomide in human glioma cells by regulating miR-138-5p/SOX9 axis. Hum Cell. 2019; 33(1):159-174. DOI: 10.1007/s13577-019-00281-1. View

Zhang S, Jiang H, Xu Z, Jiang Y, She Y, Huang X . The resistance of esophageal cancer cells to paclitaxel can be reduced by the knockdown of long noncoding RNA DDX11-AS1 through TAF1/TOP2A inhibition. Am J Cancer Res. 2019; 9(10):2233-2248. PMC: 6834486. View

Wu L, Liu Y, Guo C, Shao Y . LncRNA OIP5-AS1 promotes the malignancy of pancreatic ductal adenocarcinoma via regulating miR-429/FOXD1/ERK pathway. Cancer Cell Int. 2020; 20:296. PMC: 7346488. DOI: 10.1186/s12935-020-01366-w. View

Gusyatiner O, Hegi M . Glioma epigenetics: From subclassification to novel treatment options. Semin Cancer Biol. 2017; 51:50-58. DOI: 10.1016/j.semcancer.2017.11.010. View

Jin M, Ren J, Luo M, You Z, Fang Y, Han Y . Long non-coding RNA JPX correlates with poor prognosis and tumor progression in non-small-cell lung cancer by interacting with miR-145-5p and CCND2. Carcinogenesis. 2019; 41(5):634-645. DOI: 10.1093/carcin/bgz125. View

Song W, Qian Y, Zhang M, Wang H, Wen X, Yang X . The long non-coding RNA DDX11-AS1 facilitates cell progression and oxaliplatin resistance via regulating miR-326/IRS1 axis in gastric cancer. Eur Rev Med Pharmacol Sci. 2020; 24(6):3049-3061. DOI: 10.26355/eurrev_202003_20669. View

10.

Wang H, Wu J, Guo W . SP1-Mediated Upregulation of lncRNA LINC01614 Functions a ceRNA for miR-383 to Facilitate Glioma Progression Through Regulation of ADAM12. Onco Targets Ther. 2020; 13:4305-4318. PMC: 7244248. DOI: 10.2147/OTT.S242854. View

11.

Wu L, Zhu X, Song Z, Chen D, Guo M, Liang J . Long Non-Coding RNA HOXA-AS2 Enhances The Malignant Biological Behaviors In Glioma By Epigenetically Regulating RND3 Expression. Onco Targets Ther. 2019; 12:9407-9419. PMC: 6844264. DOI: 10.2147/OTT.S225678. View

12.

Yan Z, Zhang W, Xiong Y, Wang Y, Li Z . Long noncoding RNA FLVCR1-AS1 aggravates biological behaviors of glioma cells via targeting miR-4731-5p/E2F2 axis. Biochem Biophys Res Commun. 2019; 521(3):716-720. DOI: 10.1016/j.bbrc.2019.10.106. View

13.

Hou Y . MiR-506 inhibits cell proliferation, invasion, migration and epithelial-to-mesenchymal transition through targeting RWDD4 in human bladder cancer. Oncol Lett. 2019; 17(1):73-78. PMC: 6313135. DOI: 10.3892/ol.2018.9594. View

14.

Ouyang Q, Cui Y, Yang S, Wei W, Zhang M, Zeng J . lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis. Onco Targets Ther. 2020; 13:5033-5046. PMC: 7280253. DOI: 10.2147/OTT.S241503. View

15.

Han B, Ge Y, Cui J, Liu B . Down-regulation of lncRNA DNAJC3-AS1 inhibits colon cancer via regulating miR-214-3p/LIVIN axis. Bioengineered. 2020; 11(1):524-535. PMC: 7202691. DOI: 10.1080/21655979.2020.1757224. View

16.

Lian Y, Yan C, Lian Y, Yang R, Chen Q, Ma D . Long intergenic non-protein-coding RNA 01446 facilitates the proliferation and metastasis of gastric cancer cells through interacting with the histone lysine-specific demethylase LSD1. Cell Death Dis. 2020; 11(7):522. PMC: 7351757. DOI: 10.1038/s41419-020-2729-0. View

17.

Liu B, Ye B, Yang L, Zhu X, Huang G, Zhu P . Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression. Nat Immunol. 2017; 18(5):499-508. DOI: 10.1038/ni.3712. View

18.

Rizzo D, Ruggiero A, Martini M, Rizzo V, Maurizi P, Riccardi R . Molecular Biology in Pediatric High-Grade Glioma: Impact on Prognosis and Treatment. Biomed Res Int. 2015; 2015:215135. PMC: 4584033. DOI: 10.1155/2015/215135. View

19.

Wen P, Kesari S . Malignant gliomas in adults. N Engl J Med. 2008; 359(5):492-507. DOI: 10.1056/NEJMra0708126. View

20.

Yin G, Tian P, Buhe A, Yan W, Li T, Sun Z . LncRNA LINC00689 Promotes the Progression of Gastric Cancer Through Upregulation of ADAM9 by Sponging miR-526b-3p. Cancer Manag Res. 2020; 12:4227-4239. PMC: 7280092. DOI: 10.2147/CMAR.S231042. View