Pectin-Lyase-Modified Ginseng Extract and Ginsenoside Rd Inhibits High Glucose-Induced ROS Production in Mesangial Cells and Prevents Renal Dysfunction in Db/db Mice

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jan 15

PMID 33445772

Citations 3

Authors

Eunsoo Jung

Mi-Kyung Pyo

JungHyun Kim

Affiliations

Soon will be listed here.

Abstract

Diabetes increases the incidence rate of chronic renal disease. Pectin-lyase-modified ginseng (GS-E3D), with enhanced ginsenoside Rd content, has been newly developed. In this study, renal protective roles of GS-E3D in type-2 diabetic db/db mice were investigated. The generation of reactive oxygen species (ROS) induced by high glucose (25 mM) was reduced by ES-E3D (75%) and ginsenoside Rd (60%). Diabetic db/db mice received 100 or 250 mg/kg/day of GS-E3D daily via oral gavage for 6 weeks. Albuminuria and urinary 8-hydroxy-2'-deoxyguanosine (8-OhdG, an oxidative stress marker) levels were increased in db/db mice and the levels recovered after GS-E3D treatment. In renal tissues, TUNEL-positive cells were decreased after GS-E3D treatment, and the increased apoptosis-related protein expressions were restored after GS-E3D treatment. Therefore, GS-E3D has a potent protective role in diabetes-induced renal dysfunction through antioxidative and antiapoptotic activities. These results may help patients to select a dietary supplement for diabetes when experiencing renal dysfunction.

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References

Song Y, Cook N, Albert C, Van Denburgh M, Manson J . Effects of vitamins C and E and beta-carotene on the risk of type 2 diabetes in women at high risk of cardiovascular disease: a randomized controlled trial. Am J Clin Nutr. 2009; 90(2):429-37. PMC: 2848361. DOI: 10.3945/ajcn.2009.27491. View

Park C, Pyo M, Lee H, Hong S, Kim S, Park C . Acute and genetic toxicity of GS-E3D, a new pectin lyase-modified red ginseng extract. Regul Toxicol Pharmacol. 2019; 104:157-162. DOI: 10.1016/j.yrtph.2019.03.010. View

Van J, Scholey J, Konvalinka A . Insights into Diabetic Kidney Disease Using Urinary Proteomics and Bioinformatics. J Am Soc Nephrol. 2017; 28(4):1050-1061. PMC: 5373465. DOI: 10.1681/ASN.2016091018. View

Desagher S, Martinou J . Mitochondria as the central control point of apoptosis. Trends Cell Biol. 2000; 10(9):369-77. DOI: 10.1016/s0962-8924(00)01803-1. View

Allen D, Yaqoob M, Harwood S . Mechanisms of high glucose-induced apoptosis and its relationship to diabetic complications. J Nutr Biochem. 2005; 16(12):705-13. DOI: 10.1016/j.jnutbio.2005.06.007. View

Ghamarian A, Abdollahi M, Su X, Amiri A, Ahadi A, Nowrouzi A . Effect of chicory seed extract on glucose tolerance test (GTT) and metabolic profile in early and late stage diabetic rats. Daru. 2013; 20(1):56. PMC: 3556018. DOI: 10.1186/2008-2231-20-56. View

Susztak K, Raff A, Schiffer M, Bottinger E . Glucose-induced reactive oxygen species cause apoptosis of podocytes and podocyte depletion at the onset of diabetic nephropathy. Diabetes. 2005; 55(1):225-33. View

Hong S, Suk K, Choi S, Lee J, Sung H, Kim C . Anti-oxidant and natural killer cell activity of Korean red ginseng (Panax ginseng) and urushiol (Rhus vernicifera Stokes) on non-alcoholic fatty liver disease of rat. Food Chem Toxicol. 2013; 55:586-91. DOI: 10.1016/j.fct.2013.01.022. View

Tchamgoue A, Tchokouaha L, Tsabang N, Tarkang P, Kuiate J, Agbor G . Protects Cardio-, Hepato-, and Reno-Antioxidant Status in Streptozotocin-Intoxicated Wistar Rats. Biomed Res Int. 2019; 2018:4907648. PMC: 6286743. DOI: 10.1155/2018/4907648. View

10.

Dounousi E, Duni A, Leivaditis K, Vaios V, Eleftheriadis T, Liakopoulos V . Improvements in the Management of Diabetic Nephropathy. Rev Diabet Stud. 2015; 12(1-2):119-33. PMC: 5397987. DOI: 10.1900/RDS.2015.12.119. View

11.

Kim C, Jo K, Kim J, Pyo M, Kim J . GS-E3D, a new pectin lyase-modified red ginseng extract, inhibited diabetes-related renal dysfunction in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2017; 17(1):430. PMC: 5576329. DOI: 10.1186/s12906-017-1925-7. View

12.

Kim G, Pyo M, Chung S . Pectin lyase-modified red ginseng extract improves glucose homeostasis in high fat diet-fed mice. J Ethnopharmacol. 2019; 249:112384. DOI: 10.1016/j.jep.2019.112384. View

13.

Li H, Wang F, Zhang L, Cao Y, Liu W, Hao J . Modulation of Nrf2 expression alters high glucose-induced oxidative stress and antioxidant gene expression in mouse mesangial cells. Cell Signal. 2011; 23(10):1625-32. DOI: 10.1016/j.cellsig.2011.05.016. View

14.

Piccoli G, Grassi G, Cabiddu G, Nazha M, Roggero S, Capizzi I . Diabetic Kidney Disease: A Syndrome Rather Than a Single Disease. Rev Diabet Stud. 2015; 12(1-2):87-109. PMC: 5397985. DOI: 10.1900/RDS.2015.12.87. View

15.

Shukla R, Banerjee S, Tripathi Y . Antioxidant and Antiapoptotic effect of aqueous extract of Pueraria tuberosa (Roxb. Ex Willd.) DC. On streptozotocin-induced diabetic nephropathy in rats. BMC Complement Altern Med. 2018; 18(1):156. PMC: 5948837. DOI: 10.1186/s12906-018-2221-x. View

16.

Park H, Kim S, Mun A, Go H, Kim G, Kim S . Korean red ginseng and its primary ginsenosides inhibit ethanol-induced oxidative injury by suppression of the MAPK pathway in TIB-73 cells. J Ethnopharmacol. 2012; 141(3):1071-6. DOI: 10.1016/j.jep.2012.03.038. View

17.

Yokozawa T, Iwano M, Dohi K, Hattori M, Oura H . Inhibitory effects of ginseng on proliferation of cultured mouse mesangial cells. Nihon Jinzo Gakkai Shi. 1994; 36(1):13-8. View

18.

Yokozawa T, Owada S . Effect of ginsenoside-Rd in cephaloridine-induced renal disorder. Nephron. 1999; 81(2):200-7. DOI: 10.1159/000045277. View

19.

Nishikawa T, Edelstein D, Du X, Yamagishi S, Matsumura T, Kaneda Y . Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature. 2000; 404(6779):787-90. DOI: 10.1038/35008121. View

20.

White K, Bilous R . Type 2 diabetic patients with nephropathy show structural-functional relationships that are similar to type 1 disease. J Am Soc Nephrol. 2000; 11(9):1667-1673. DOI: 10.1681/ASN.V1191667. View