Protein N-glycosylation and O-mannosylation Are Catalyzed by Two Evolutionarily Related GT-C Glycosyltransferases

Overview

Journal Curr Opin Struct Biol

Date 2021 Jan 14

PMID 33445129

Citations 3

Authors

Lin Bai

Huilin Li

Affiliations

Soon will be listed here.

Abstract

The structural folds of glycosyltransferases are categorized into three superfamilies: GT-A, GT-B, and GT-C. Few structures of GT-C fold existed in the Protein Data Bank prior to the recent advent of high-resolution cryo-EM, because the glycosyltransferases are large membrane proteins that are difficult to crystallize. The use of cryo-EM has resulted in the structures of several key GT-C glycosyltransferases. Here we summarize the latest structural features of and mechanistic insights into these membrane enzyme complexes.

Citing Articles

The oligosaccharyltransferase subunit PsSTT3A regulates N-glycosylation and is critical for development and virulence of Phytophthora sojae.

Cui T, Ma Q, Zhang F, Chen S, Zhang C, Zhou X Sci China Life Sci. 2025; .

PMID: 40059268 DOI: 10.1007/s11427-024-2807-y.

Unveiling the GT114 family: Structural characterization of A075L, a glycosyltransferase from Paramecium bursaria chlorella virus-1 (PBCV-1).

Laugieri M, Speciale I, Gimeno A, Lin S, Byers B, Poveda A Protein Sci. 2024; 33(12):e5196.

PMID: 39555664 PMC: 11571054. DOI: 10.1002/pro.5196.

Protein O-mannosylation: one sugar, several pathways, many functions.

Koff M, Monagas-Valentin P, Novikov B, Chandel I, Panin V Glycobiology. 2023; 33(11):911-926.

PMID: 37565810 PMC: 10859634. DOI: 10.1093/glycob/cwad067.

Sculpting the Bacterial -Glycoproteome: Functional Analyses of Orthologous Oligosaccharyltransferases with Diverse Targeting Specificities.

Hadjineophytou C, Anonsen J, Svingerud T, Mortimer T, Grad Y, Scott N mBio. 2022; 13(3):e0379721.

PMID: 35471082 PMC: 9239064. DOI: 10.1128/mbio.03797-21.

References

Cherepanova N, Shrimal S, Gilmore R . N-linked glycosylation and homeostasis of the endoplasmic reticulum. Curr Opin Cell Biol. 2016; 41:57-65. PMC: 4983500. DOI: 10.1016/j.ceb.2016.03.021. View

Loibl M, Strahl S . Protein O-mannosylation: what we have learned from baker's yeast. Biochim Biophys Acta. 2013; 1833(11):2438-46. DOI: 10.1016/j.bbamcr.2013.02.008. View

Pfeffer S, Burbaum L, Unverdorben P, Pech M, Chen Y, Zimmermann R . Structure of the native Sec61 protein-conducting channel. Nat Commun. 2015; 6:8403. PMC: 4598622. DOI: 10.1038/ncomms9403. View

Zhang L, Zhao Y, Gao R, Li J, Yang X, Gao Y . Cryo-EM snapshots of mycobacterial arabinosyltransferase complex EmbB-AcpM. Protein Cell. 2020; 11(7):505-517. PMC: 7305291. DOI: 10.1007/s13238-020-00726-6. View

Lairson L, Henrissat B, Davies G, Withers S . Glycosyltransferases: structures, functions, and mechanisms. Annu Rev Biochem. 2008; 77:521-55. DOI: 10.1146/annurev.biochem.76.061005.092322. View

Maeda Y, Kinoshita T . Dolichol-phosphate mannose synthase: structure, function and regulation. Biochim Biophys Acta. 2008; 1780(6):861-8. DOI: 10.1016/j.bbagen.2008.03.005. View

Helenius A, Aebi M . Roles of N-linked glycans in the endoplasmic reticulum. Annu Rev Biochem. 2004; 73:1019-49. DOI: 10.1146/annurev.biochem.73.011303.073752. View

Bai L, Kovach A, You Q, Kenny A, Li H . Structure of the eukaryotic protein O-mannosyltransferase Pmt1-Pmt2 complex. Nat Struct Mol Biol. 2019; 26(8):704-711. PMC: 6684406. DOI: 10.1038/s41594-019-0262-6. View

Cherepanova N, Shrimal S, Gilmore R . Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins. J Cell Biol. 2014; 206(4):525-39. PMC: 4137057. DOI: 10.1083/jcb.201404083. View

10.

Schwarz F, Aebi M . Mechanisms and principles of N-linked protein glycosylation. Curr Opin Struct Biol. 2011; 21(5):576-82. DOI: 10.1016/j.sbi.2011.08.005. View

11.

Bai L, Li H . Cryo-EM is uncovering the mechanism of eukaryotic protein N-glycosylation. FEBS J. 2018; 286(9):1638-1644. PMC: 6504584. DOI: 10.1111/febs.14705. View

12.

Aebi M . N-linked protein glycosylation in the ER. Biochim Biophys Acta. 2013; 1833(11):2430-7. DOI: 10.1016/j.bbamcr.2013.04.001. View

13.

Nakane T, Kotecha A, Sente A, McMullan G, Masiulis S, Brown P . Single-particle cryo-EM at atomic resolution. Nature. 2020; 587(7832):152-156. PMC: 7611073. DOI: 10.1038/s41586-020-2829-0. View

14.

Matsumoto S, Shimada A, Nyirenda J, Igura M, Kawano Y, Kohda D . Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation. Proc Natl Acad Sci U S A. 2013; 110(44):17868-73. PMC: 3816453. DOI: 10.1073/pnas.1309777110. View

15.

Peplow M . Cryo-Electron Microscopy Reaches Resolution Milestone. ACS Cent Sci. 2020; 6(8):1274-1277. PMC: 7453411. DOI: 10.1021/acscentsci.0c01048. View

16.

Tan Y, Zhang L, Rodrigues J, Zheng R, Giacometti S, Rosario A . Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria. Mol Cell. 2020; 78(4):683-699.e11. PMC: 7263364. DOI: 10.1016/j.molcel.2020.04.014. View

17.

Matsumoto S, Taguchi Y, Shimada A, Igura M, Kohda D . Tethering an N-Glycosylation Sequon-Containing Peptide Creates a Catalytically Competent Oligosaccharyltransferase Complex. Biochemistry. 2016; 56(4):602-611. DOI: 10.1021/acs.biochem.6b01089. View

18.

Braunger K, Pfeffer S, Shrimal S, Gilmore R, Berninghausen O, Mandon E . Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum. Science. 2018; 360(6385):215-219. PMC: 6319373. DOI: 10.1126/science.aar7899. View

19.

Li H, Chavan M, Schindelin H, Lennarz W, Li H . Structure of the oligosaccharyl transferase complex at 12 A resolution. Structure. 2008; 16(3):432-40. DOI: 10.1016/j.str.2007.12.013. View

20.

Napiorkowska M, Boilevin J, Sovdat T, Darbre T, Reymond J, Aebi M . Molecular basis of lipid-linked oligosaccharide recognition and processing by bacterial oligosaccharyltransferase. Nat Struct Mol Biol. 2017; 24(12):1100-1106. DOI: 10.1038/nsmb.3491. View