» Articles » PMID: 33441341

Cross-Reactive Anti-Nucleocapsid Protein Immunity Against Crimean-Congo Hemorrhagic Fever Virus and Hazara Virus in Multiple Species

Overview
Journal J Virol
Date 2021 Jan 14
PMID 33441341
Citations 14
Authors
Affiliations
Soon will be listed here.
Abstract

The World Health Organization estimates that there may be three billion people at risk of infection by Crimean-Congo Hemorrhagic Fever Virus (CCHFV), a highly lethal, emerging carried by ticks. On the other hand, the closely related Hazara virus (HAZV), a member of the same serogroup, has not been reported as a pathogen for humans. Given the structural and phylogenetic similarities between these two viruses, we evaluated the immunological similarities of the nucleocapsid protein (NP) of these two viruses in multiple species. Strong antigenic similarities were demonstrated in anti-NP humoral immune responses against HAZV and CCHFV in multiple species using convalescent human CCHF sera, rabbit and mouse polyclonal antiserum raised against CCHFV, and mouse polyclonal antiserum against CCHFV-NP in enzyme immunoassays. We also report a convincing cross-reactivity between NPs in Western blots using HAZV-infected cell lysate as antigen and inactivated CCHFV and CCHFV-NP-immunized mice sera. These results suggest that NPs of HAZV and CCHFV share significant similarities in humoral responses across species and underline the potential utility of HAZV as a surrogate model for CCHFV. CCHFV and HAZV, members of the family, are transmitted to mammals by tick bites. CCHFV is considered to be a severe threat to public health and causes hemorrhagic diseases with a high mortality rate, and there are neither preventative nor therapeutic medications against CCHFV disease. HAZV, on the other hand, is not a pathogen to humans and can be studied under BSL-2 conditions. The antigenic relationship between these viruses is of interest for vaccines and for preventative investigations. Here, we demonstrate cross-reactivity in anti-NP humoral immune response between NPs of HAZV and CCHFV in multiple species. These results underline the utility of HAZV as a surrogate model to study CCHFV infection.

Citing Articles

The Role of Nucleocapsid Protein (NP) in the Immunology of Crimean-Congo Hemorrhagic Fever Virus (CCHFV).

Pirincal A, Doymaz M Viruses. 2024; 16(10).

PMID: 39459881 PMC: 11512346. DOI: 10.3390/v16101547.


Cellular immunity to nucleoproteins (NP) of Crimean-Congo hemorrhagic fever virus (CCHFV) and Hazara Virus (HAZV).

Kalkan-Yazici M, Karaaslan E, Guler-Cetin N, Doymaz M Med Microbiol Immunol. 2024; 213(1):20.

PMID: 39320473 DOI: 10.1007/s00430-024-00802-2.


Crimean Congo Hemorrhagic Fever Virus for Clinicians-Virology, Pathogenesis, and Pathology.

Frank M, Weaver G, Raabe V Emerg Infect Dis. 2024; 30(5):847-853.

PMID: 38666566 PMC: 11060449. DOI: 10.3201/eid3005.231646.


The Adaptive Immune Response against .

Alatrash R, Herrera B Viruses. 2024; 16(3).

PMID: 38543848 PMC: 10974645. DOI: 10.3390/v16030483.


Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana.

Addo S, Bentil R, Addae C, Baako B, Yartey K, Ansah-Owusu J Arch Virol. 2024; 169(3):62.

PMID: 38446223 DOI: 10.1007/s00705-024-05983-y.


References
1.
Surtees R, Dowall S, Shaw A, Armstrong S, Hewson R, Carroll M . Heat Shock Protein 70 Family Members Interact with Crimean-Congo Hemorrhagic Fever Virus and Hazara Virus Nucleocapsid Proteins and Perform a Functional Role in the Nairovirus Replication Cycle. J Virol. 2016; 90(20):9305-16. PMC: 5044845. DOI: 10.1128/JVI.00661-16. View

2.
Zhou H, Sun Y, Guo Y, Lou Z . Structural perspective on the formation of ribonucleoprotein complex in negative-sense single-stranded RNA viruses. Trends Microbiol. 2013; 21(9):475-84. DOI: 10.1016/j.tim.2013.07.006. View

3.
Matsumoto Y, Ohta K, Nishio M . Lethal infection of embryonated chicken eggs by Hazara virus, a model for Crimean-Congo hemorrhagic fever virus. Arch Virol. 2017; 163(1):219-222. DOI: 10.1007/s00705-017-3580-1. View

4.
Ding B, Qin Y, Chen M . Nucleocapsid proteins: roles beyond viral RNA packaging. Wiley Interdiscip Rev RNA. 2016; 7(2):213-26. PMC: 7169677. DOI: 10.1002/wrna.1326. View

5.
Emmerich P, Mika A, von Possel R, Rackow A, Liu Y, Schmitz H . Sensitive and specific detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-Specific IgM and IgG antibodies in human sera using recombinant CCHFV nucleoprotein as antigen in μ-capture and IgG immune complex (IC) ELISA tests. PLoS Negl Trop Dis. 2018; 12(3):e0006366. PMC: 5892944. DOI: 10.1371/journal.pntd.0006366. View