The Acquisition of Colistin Resistance Is Associated to the Amplification of a Large Chromosomal Region in Kp52145

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Jan 14

PMID 33440735

Citations 2

Authors

Maria Blanca Sanchez

Alicia Sanchez-Gorostiaga

Trinidad Cuesta

Jose Luis Martinez

Affiliations

Soon will be listed here.

Abstract

The appearance of carbapenem-resistant has increased the use of colistin as a last-resort antibiotic for treating infections by this pathogen. A consequence of its use has been the spread of colistin-resistant strains, in several cases carrying colistin resistance genes. In addition, when susceptible strains are confronted with colistin during treatment, mutation is a major cause of the acquisition of resistance. To analyze the mechanisms of resistance that might be selected during colistin treatment, an experimental evolution assay for 30 days using as a model the clinical kp52145 isolate in the presence of increasing amounts of colistin was performed. All evolved populations presented a decreased susceptibility to colistin, without showing cross-resistance to antibiotics belonging to other structural families. We did not find any common mutation in the evolved mutants, neither in already known genes, previously known to be associated with the resistance phenotype, nor in new ones. The only common genetic change observed in the strains that evolved in the presence of colistin was the amplification of a 34 Kb sequence, homologous to a prophage (Enterobacteria phage Fels-2). Our data support that gene amplification can be a driving force in the acquisition of colistin resistance by .

Citing Articles

High prevalence of polymyxin-heteroresistant carbapenem-resistant Klebsiella pneumoniae and its within-host evolution to resistance among critically ill scenarios.

Wang X, Meng T, Dai Y, Ou H, Wang M, Tang B Infection. 2024; 53(1):271-283.

PMID: 39143437 PMC: 11825540. DOI: 10.1007/s15010-024-02365-z.

Prevalence of Mutated Colistin-Resistant : A Systematic Review and Meta-Analysis.

Yusof N, Norazzman N, Hakim S, Mohd Azlan M, Amilda Anthony A, Mustafa F Trop Med Infect Dis. 2022; 7(12).

PMID: 36548669 PMC: 9782491. DOI: 10.3390/tropicalmed7120414.

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