Random Access Parallel Microscopy

Overview

Journal Elife

Specialty Biology

Date 2021 Jan 12

PMID 33432922

Citations 9

Authors

Mishal Ashraf

Sharika Mohanan

Byu Ri Sim

Anthony Tam

Kiamehr Rahemipour

Denis Brousseau

Simon Thibault

Alexander D Corbett

Gil Bub

Affiliations

Soon will be listed here.

Abstract

We introduce a random-access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long-duration records from different samples in parallel, which is not possible using conventional automated microscopes. The system is demonstrated by continuously imaging multiple cardiac monolayer and preparations.

Citing Articles

Three-dimensional random-access confocal microscopy with 3D remote focusing system.

Li H, Lu Q, Wang Z, Zhang W, Wu Y, Sun Y Commun Eng. 2024; 3(1):166.

PMID: 39528669 PMC: 11555065. DOI: 10.1038/s44172-024-00320-2.

OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology.

Heinson Y, Han J, Entcheva E J Mol Cell Cardiol Plus. 2023; 6.

PMID: 38130942 PMC: 10735237. DOI: 10.1016/j.jmccpl.2023.100054.

OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology.

Heinson Y, Han J, Entcheva E bioRxiv. 2023; .

PMID: 37693544 PMC: 10491208. DOI: 10.1101/2023.08.29.555447.

Simultaneous Widefield Voltage and Dye-Free Optical Mapping Quantifies Electromechanical Waves in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Liu W, Han J, Tomek J, Bub G, Entcheva E ACS Photonics. 2023; 10(4):1070-1083.

PMID: 37096210 PMC: 10119986. DOI: 10.1021/acsphotonics.2c01644.

Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology.

Heinson Y, Han J, Entcheva E J Biomed Opt. 2023; 28(1):016001.

PMID: 36636698 PMC: 9830584. DOI: 10.1117/1.JBO.28.1.016001.

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