Interaction Study of Pasteurella Multocida with Culturable Aerobic Bacteria Isolated from Porcine Respiratory Tracts Using Coculture in Conditioned Media

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2021 Jan 10

PMID 33422011

Citations 2

Authors

Nonzee Hanchanachai

Pramote Chumnanpuen

Teerasak E-Kobon

Affiliations

Soon will be listed here.

Abstract

Background: The porcine respiratory tract harbours multiple microorganisms, and the interactions between these organisms could be associated with animal health status. Pasteurella multocida is a culturable facultative anaerobic bacterium isolated from healthy and diseased porcine respiratory tracts. The interaction between P. multocida and other aerobic commensal bacteria in the porcine respiratory tract is not well understood. This study aimed to determine the interactions between porcine P. multocida capsular serotype A and D strains and other culturable aerobic bacteria isolated from porcine respiratory tracts using a coculture assay in conditioned media followed by calculation of the growth rates and interaction parameters.

Results: One hundred and sixteen bacterial samples were isolated from five porcine respiratory tracts, and 93 isolates were identified and phylogenetically classified into fourteen genera based on 16S rRNA sequences. Thirteen isolates from Gram-negative bacterial genera and two isolates from the Gram-positive bacterial genus were selected for coculture with P. multocida. From 17 × 17 (289) interaction pairs, the majority of 220 pairs had negative interactions indicating competition for nutrients and space, while 17 pairs were identified as mild cooperative or positive interactions indicating their coexistence. All conditioned media, except those of Acinetobacter, could inhibit P. multocida growth. Conversely, the conditioned media of P. multocida also inhibited the growth of nine isolates plus themselves.

Conclusion: Negative interaction was the major interactions among the coculture of these 15 representative isolates and the coculture with P. multocida. The conditioned media in this study might be further analysed to identify critical molecules and examined by the in vivo experiments. The study proposed the possibility of using these molecules in conditioned media to control P. multocida growth.

Citing Articles

Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019-2021.

Xu X, Li J, Huang P, Cui X, Li X, Sun J Vet Sci. 2023; 10(8).

PMID: 37624289 PMC: 10458188. DOI: 10.3390/vetsci10080502.

Effect of conditioned media from Aeromonas caviae on the transcriptomic changes of the porcine isolates of Pasteurella multocida.

Hanchanachai N, Chumnanpuen P, E-Kobon T BMC Microbiol. 2022; 22(1):272.

PMID: 36368971 PMC: 9650167. DOI: 10.1186/s12866-022-02683-y.

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