The Mechanism of Action of GTP on Ca2+ Efflux from Rat Liver Microsomal Vesicles. Measurement of Vesicle Fusion by Fluorescence Energy Transfer

Overview

Journal Biochem J

Specialty Biochemistry

Date 1988 Jan 1

PMID 3342018

Citations 9

Authors

J G Comerford

A P Dawson

Affiliations

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Abstract

1. GTP-promoted fusion between microsomal vesicles was studied by using fluorescence-resonance-energy transfer between the fluorescent membrane probes octadecanoyl-aminofluorescein and octadecyl-rhodamine. 2. The fluorescence increase after GTP addition does not require the presence of ATP, is unaffected by changes in free [Ca2+] in the range 10 microM-1 nM, but requires Mg2+, although higher Mg2+ concentrations are inhibitory. 3. In terms of requirements for poly(ethylene glycol), dependence on GTP concentration and inhibition by high Mg2+ concentrations, there is excellent correlation between rate of increase in fluorescence and rate of GTP-promoted Ca2+ efflux measured under Ca2+ transport conditions. 4. The observations support our previous conclusions that GTP-induced membrane fusion plays a major role in causing GTP-promoted Ca2+ efflux from microsomal vesicles.

Citing Articles

Effects of CoA and acyl-CoAs on GTP-dependent Ca2+ release and vesicle fusion in rat liver microsomal vesicles.

Comerford J, Dawson A Biochem J. 1993; 289 ( Pt 2):561-7.

PMID: 8380999 PMC: 1132205. DOI: 10.1042/bj2890561.

Effect of GTP on the dolichol pathway for protein glycosylation in rat liver microsomes.

Bossuyt X, Blanckaert N Biochem J. 1993; 296 ( Pt 3):633-7.

PMID: 8280061 PMC: 1137744. DOI: 10.1042/bj2960633.

Effects of CoA and acyl-CoA on Ca(2+)-permeability of endoplasmic-reticulum membranes from rat liver.

RICH G, Comerford J, Graham S, Dawson A Biochem J. 1995; 306 ( Pt 3):703-8.

PMID: 7702563 PMC: 1136578. DOI: 10.1042/bj3060703.

Detection of GTP-binding proteins in purified derivatives of rough endoplasmic reticulum.

Lanoix J, Roy L, Paiement J Biochem J. 1989; 262(2):497-503.

PMID: 2508629 PMC: 1133296. DOI: 10.1042/bj2620497.

The effect of limited proteolysis on GTP-dependent Ca2+ efflux and GTP-dependent fusion in rat liver microsomal vesicles.

Comerford J, Dawson A Biochem J. 1989; 258(3):823-9.

PMID: 2499309 PMC: 1138438. DOI: 10.1042/bj2580823.

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