Structural Characterization and Antioxidant Activity of an Acetylated Cyclocarya Paliurus Polysaccharide (Ac-CPP)

The aim of this study was to investigate the primary structure of an acetylated Cyclocarya paliurus polysaccharide (Ac-CPP) and its protective effect on HO-treated dendritic cells. The backbone of Ac-CPP was →3)-β-D-Galp-(1→, with some branches α-L-Araf-(1→ residues at O-6 and O-5, β-D-Galp-(1→ and 3,5,6)-β-D-Galf-(1 residues at O-4 and acetyl groups were substituted at the O-2 and O-6 positions of 3)-β-D-Galp-(1 residues. The CPP and Ac-CPP significantly increased the levels of superoxide dismutase, glutathione peroxidase and catalase on HO-treated dendritic cells. Meanwhile, both CPP and Ac-CPP up-regulated the expression of Nrf2 (NF-E2-related factor 2) and down-regulated the Keap1 (Kelch-like ECH-associated protein-1), but Ac-CPP had a better effect on antioxidant capacity. These results indicated that potential application of Ac-CPP as an antioxidant agent.