Skin-Related Properties and Constituents from the Aerial Parts Extract of

In the course of screening for cosmetic ingredients by measuring antioxidant and antiwrinkle and whitening and anti-inflammatory activities, skin-related activity was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, elastase inhibition, tyrosinase inhibition, and nitric oxide assay. Several Polygonaseae extracts were found to show potent activity. The results showed that the methanolic extract has the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ABTS radical scavenging activities (IC 61.0 and 17.5 g/mL). In the elastase inhibition assay and nitric oxide assay, the IC of methanolic extract of was 739.7 g/mL and 71.8 g/mL. The 70% ethanolic extract partitioned with n-hexane, CHCl, EtOAc, n-BuOH, and aqueous fractions. The purification of EtOAc soluble layer was by column chromatography separation and MPLC analysis of Compounds . It was identified as loliolide (), quercetin-3-O-glucoside (), quercetin-3-O-glucuronide (), 4-methoxy caftraric acid (), kaempferol-3-(6-methylglucuronide) (), quercetin-3-(6-methylglucuronide) (), and quercetin (). Structure was elucidated by a combination of 1D and 2D NMR and MS spectrometry as well as comparison with reported literatures. Radical scavenging effect on DPPH, tyrosinase inhibition, and nitric oxide assay on several compounds from was found to show potent activity. The results showed that Compound has the NO assay (IC29.7 M). For DPPH, the IC of Compounds , , , and was 39.6, 31.2, 37.0, and 22.7 M. In tyrosinase inhibitory activity, the IC of Compound was 14.3 M.