LncRNA NORAD Promotes Bone Marrow Stem Cell Differentiation and Proliferation by Targeting MiR-26a-5p in Steroid-induced Osteonecrosis of the Femoral Head

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2021 Jan 8

PMID 33413642

Citations 12

Authors

Dapeng Fu

Sheng Yang

Jianmin Lu

Haoyi Lian

Kairong Qin

Affiliations

Soon will be listed here.

Abstract

Background: Steroid-induced osteonecrosis of the femoral head (SONFH) is a devastating orthopedic disease, which seriously affects the quality of life of patients. The study aims to investigate the effects of LncRNA NORAD on SONFH.

Methods: Human bone marrow-derived mesenchymal stem cells (hBMSCs) were isolated from the proximal femur of patients during routine orthopedic surgery and then cultured with dexamethasone (Dex) and transfected with NORAD overexpression vector, siRNA-NORAD and miR-26a-5p mimics. The mRNA expression of NORAD, miR-26a-5p, OPG, RANK, and RANKL was detected by RT-qPCR. Cell proliferation and apoptosis was measured by CCK-8 assay and flow cytometry, respectively. The protein expression of RUNX2, OPG, RANK, and RANKL was detected by western blot. The dual-luciferase reporter gene assay was performed to confirm the binding between NORAD and miR-26a-5p.

Results: NORAD expression was downregulated in SONFH tissues, while miR-26a-5p expression was upregulated. Overexpression of NORAD improved DEX-induced inhibition of proliferation and differentiation, and promotion of apoptosis in hBMSCs, while knockdown of NORAD led to the opposite results. Moreover, NORAD improved DEX-induced inhibition of proliferation and differentiation, and promotion of apoptosis by regulation of miR-26a-5p in hBMSCs.

Conclusions: NORAD expression was downregulated in SONFH tissues, while miR-26a-5p expression was upregulated. NORAD improved DEX-induced inhibition of proliferation and differentiation, and promotion of apoptosis by regulation of miR-26a-5p in hBMSCs.

Citing Articles

Bioinformatics and Gene Expression Omnibus Analysis of Key Candidate Genes and Pathways Associated with Femoral Head Necrosis.

Ding Y, Ma Y, Yan H, Zhang E Iran J Pharm Res. 2025; 23(1):e145223.

PMID: 39830663 PMC: 11742569. DOI: 10.5812/ijpr-145223.

Insight into Steroid-Induced ONFH: The Molecular Mechanism and Function of Epigenetic Modification in Mesenchymal Stem Cells.

Huang C, Qing L, Xiao Y, Tang J, Wu P Biomolecules. 2024; 14(1).

PMID: 38275745 PMC: 10813482. DOI: 10.3390/biom14010004.

Long Noncoding RNA NORAD Promotes Fracture Healing through Interacting with Osteoblast Differentiation via Targeting miR-26a.

Chen S, Ma H, Li M, Jia Z, Chen X, Bu N Biomed Res Int. 2023; 2023:9950037.

PMID: 36726840 PMC: 9886463. DOI: 10.1155/2023/9950037.

lncRNA DLX6-AS1 Promotes Myocardial Ischemia-Reperfusion Injury through Mediating the miR-204-5p/FBXW7 Axis.

Wang F, Wu Y Mediators Inflamm. 2023; 2023:9380398.

PMID: 36660176 PMC: 9845044. DOI: 10.1155/2023/9380398.

Liang X, Liu X, Li S, Wen M, Chen Y, Luo D J Orthop Surg Res. 2023; 18(1):27.

PMID: 36627660 PMC: 9832881. DOI: 10.1186/s13018-022-03381-1.

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