Structural Disruption of the Blood-brain Barrier in Repetitive Primary Blast Injury

Overview

Journal Fluids Barriers CNS

Publisher Biomed Central

Date 2021 Jan 8

PMID 33413513

Citations 6

Authors

Gozde Uzunalli

Seth Herr

Alexandra M Dieterly

Riyi Shi

L Tiffany Lyle

Affiliations

Soon will be listed here.

Abstract

Background: Blast-induced traumatic brain injury (bTBI) is a growing health concern due to the increased use of low-cost improvised explosive devices in modern warfare. Mild blast exposures are common amongst military personnel; however, these women and men typically do not have adequate recovery time from their injuries due to the transient nature of behavioral symptoms. bTBI has been linked to heterogeneous neuropathology, including brain edema, neuronal degeneration and cognitive abnormalities depending on the intensity of blast overpressure and frequency. Recent studies have reported heterogeneity in blood-brain barrier (BBB) permeability following blast injury. There still remains a limited understanding of the pathologic changes in the BBB following primary blast injuries. In this study, our goal was to elucidate the pathologic pattern of BBB damage through structural analysis following single and repetitive blast injury using a clinically relevant rat model of bTBI.

Methods: A validated, open-ended shock tube model was used to deliver single or repetitive primary blast waves. The pathology of the BBB was assessed using immunofluorescence and immunohistochemistry assays. All data were analyzed using the one-way ANOVA test.

Results: We have demonstrated that exposure to repetitive blast injury affects the desmin-positive and CD13-positive subpopulations of pericytes in the BBB. Changes in astrocytes and microglia were also detected.

Conclusion: This study provides analysis of the BBB components after repetitive blast injury. These results will be critical as preventative and therapeutic strategies are established for veterans recovering from blast-induced traumatic brain injury.

Citing Articles

Repetitive Low-Level Blast Exposure Alters Circulating Myeloperoxidase, Matrix Metalloproteinases, and Neurovascular Endothelial Molecules in Experienced Military Breachers.

Rhind S, Shiu M, Tenn C, Nakashima A, Jetly R, Sajja V Int J Mol Sci. 2025; 26(5).

PMID: 40076437 PMC: 11898641. DOI: 10.3390/ijms26051808.

Circulating Brain-Reactive Autoantibody Profiles in Military Breachers Exposed to Repetitive Occupational Blast.

Rhind S, Shiu M, Vartanian O, Tenn C, Nakashima A, Jetly R Int J Mol Sci. 2025; 25(24.

PMID: 39769446 PMC: 11728191. DOI: 10.3390/ijms252413683.

Anxiety-like Characteristics, Forepaw Thermal Sensitivity Changes and Glial Alterations 1 Month After Repetitive Blast Traumatic Brain Injury in Male Rats.

Kallakuri S, Sadik N, Davidson C, Gheidi A, Bosse K, Bir C Ann Neurosci. 2024; :09727531241248976.

PMID: 39544640 PMC: 11559877. DOI: 10.1177/09727531241248976.

Twenty Years of Blast-Induced Neurotrauma: Current State of Knowledge.

Sachdeva T, Ganpule S Neurotrauma Rep. 2024; 5(1):243-253.

PMID: 38515548 PMC: 10956535. DOI: 10.1089/neur.2024.0001.

Retinal gliosis and phenotypic diversity of intermediate filament induction and remodeling upon acoustic blast overpressure (ABO) exposure to the rat eye.

Skelton L, Ramachandra Rao S, Allen R, Motz C, Pardue M, Fliesler S Exp Eye Res. 2023; 234:109585.

PMID: 37481225 PMC: 10730083. DOI: 10.1016/j.exer.2023.109585.

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