Essential Amino Acid-enriched Whey Enhances Post-exercise Whole-body Protein Balance During Energy Deficit More Than Iso-nitrogenous Whey or a Mixed-macronutrient Meal: a Randomized, Crossover Study

Overview

Journal J Int Soc Sports Nutr

Date 2021 Jan 8

PMID 33413462

Citations 8

Authors

Jess A Gwin

David D Church

Adrienne Hatch-McChesney

Jillian T Allen

Marques A Wilson

Alyssa N Varanoske

Christopher T Carrigan

Nancy E Murphy

Lee M Margolis

John W Carbone

Robert R Wolfe

Arny A Ferrando

Stefan M Pasiakos

Affiliations

Soon will be listed here.

Abstract

Background: The effects of ingesting varying essential amino acid (EAA)/protein-containing food formats on protein kinetics during energy deficit are undetermined. Therefore, recommendations for EAA/protein food formats necessary to optimize both whole-body protein balance and muscle protein synthesis (MPS) during energy deficit are unknown. We measured protein kinetics after consuming iso-nitrogenous amounts of free-form essential amino acid-enriched whey (EAA + W; 34.7 g protein, 24 g EAA sourced from whey and free-form EAA), whey (WHEY; 34.7 g protein, 18.7 g EAA), or a mixed-macronutrient meal (MEAL; 34.7 g protein, 11.4 g EAA) after exercise during short-term energy deficit.

Methods: Ten adults (mean ± SD; 21 ± 4 y; 25.7 ± 1.7 kg/m) completed a randomized, double-blind crossover study consisting of three, 5 d energy-deficit periods (- 30 ± 3% of total energy requirements), separated by 14 d. Whole-body protein synthesis (PS), breakdown (PB), and net balance (NET) were determined at rest and in response to combination exercise consisting of load carriage treadmill walking, deadlifts, and box step-ups at the end of each energy deficit using L-[H]-phenylalanine and L-[H]-tyrosine infusions. Treatments were ingested immediately post-exercise. Mixed-muscle protein synthesis (mixed-MPS) was measured during exercise through recovery.

Results: Change (Δ postabsorptive + exercise to postprandial + recovery [mean treatment difference (95%CI)]) in whole-body (g/180 min) PS was 15.8 (9.8, 21.9; P = 0.001) and 19.4 (14.8, 24.0; P = 0.001) greater for EAA + W than WHEY and MEAL, respectively, with no difference between WHEY and MEAL. ΔPB was - 6.3 (- 11.5, - 1.18; P = 0.02) greater for EAA + W than WHEY and - 7.7 (- 11.9, - 3.6; P = 0.002) greater for MEAL than WHEY, with no difference between EAA + W and MEAL. ΔNET was 22.1 (20.5, 23.8; P = 0.001) and 18.0 (16.5, 19.5; P = 0.00) greater for EAA + W than WHEY and MEAL, respectively, while ΔNET was 4.2 (2.7, 5.6; P = 0.001) greater for MEAL than WHEY. Mixed-MPS did not differ between treatments.

Conclusions: While mixed-MPS was similar across treatments, combining free-form EAA with whey promotes greater whole-body net protein balance during energy deficit compared to iso-nitrogenous amounts of whey or a mixed-macronutrient meal.

Trial Registration: ClinicalTrials.gov, Identifier no. NCT04004715 . Retrospectively registered 28 June 2019, first enrollment 6 June 2019.

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References

Abumrad N, Rabin D, Diamond M, LACY W . Use of a heated superficial hand vein as an alternative site for the measurement of amino acid concentrations and for the study of glucose and alanine kinetics in man. Metabolism. 1981; 30(9):936-40. DOI: 10.1016/0026-0495(81)90074-3. View

Bohe J, Low A, Wolfe R, Rennie M . Human muscle protein synthesis is modulated by extracellular, not intramuscular amino acid availability: a dose-response study. J Physiol. 2003; 552(Pt 1):315-24. PMC: 2343318. DOI: 10.1113/jphysiol.2003.050674. View

Churchward-Venne T, Burd N, Mitchell C, West D, Philp A, Marcotte G . Supplementation of a suboptimal protein dose with leucine or essential amino acids: effects on myofibrillar protein synthesis at rest and following resistance exercise in men. J Physiol. 2012; 590(11):2751-65. PMC: 3424729. DOI: 10.1113/jphysiol.2012.228833. View

Park S, Church D, Azhar G, Schutzler S, Ferrando A, Wolfe R . Anabolic response to essential amino acid plus whey protein composition is greater than whey protein alone in young healthy adults. J Int Soc Sports Nutr. 2020; 17(1):9. PMC: 7011510. DOI: 10.1186/s12970-020-0340-5. View

Volpi E, Kobayashi H, Sheffield-Moore M, Mittendorfer B, Wolfe R . Essential amino acids are primarily responsible for the amino acid stimulation of muscle protein anabolism in healthy elderly adults. Am J Clin Nutr. 2003; 78(2):250-8. PMC: 3192452. DOI: 10.1093/ajcn/78.2.250. View

Nygren J, Nair K . Differential regulation of protein dynamics in splanchnic and skeletal muscle beds by insulin and amino acids in healthy human subjects. Diabetes. 2003; 52(6):1377-85. DOI: 10.2337/diabetes.52.6.1377. View

Pruessner J, Kirschbaum C, Meinlschmid G, Hellhammer D . Two formulas for computation of the area under the curve represent measures of total hormone concentration versus time-dependent change. Psychoneuroendocrinology. 2003; 28(7):916-31. DOI: 10.1016/s0306-4530(02)00108-7. View

Rutherfurd S, Fanning A, Miller B, Moughan P . Protein digestibility-corrected amino acid scores and digestible indispensable amino acid scores differentially describe protein quality in growing male rats. J Nutr. 2015; 145(2):372-9. DOI: 10.3945/jn.114.195438. View

Mifflin M, St Jeor S, Hill L, Scott B, Daugherty S, Koh Y . A new predictive equation for resting energy expenditure in healthy individuals. Am J Clin Nutr. 1990; 51(2):241-7. DOI: 10.1093/ajcn/51.2.241. View

10.

Paddon-Jones D, Sheffield-Moore M, Zhang X, Volpi E, Wolf S, Aarsland A . Amino acid ingestion improves muscle protein synthesis in the young and elderly. Am J Physiol Endocrinol Metab. 2003; 286(3):E321-8. DOI: 10.1152/ajpendo.00368.2003. View

11.

Tang J, Phillips S . Maximizing muscle protein anabolism: the role of protein quality. Curr Opin Clin Nutr Metab Care. 2008; 12(1):66-71. DOI: 10.1097/MCO.0b013e32831cef75. View

12.

Karl J, Smith T, Wilson M, Bukhari A, Pasiakos S, McClung H . Altered metabolic homeostasis is associated with appetite regulation during and following 48-h of severe energy deprivation in adults. Metabolism. 2016; 65(4):416-27. DOI: 10.1016/j.metabol.2015.11.001. View

13.

Drummond M, Fry C, Glynn E, Timmerman K, Dickinson J, Walker D . Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise. J Appl Physiol (1985). 2011; 111(1):135-42. PMC: 3137547. DOI: 10.1152/japplphysiol.01408.2010. View

14.

Volpi E, Chinkes D, Rasmussen B . Sequential muscle biopsies during a 6-h tracer infusion do not affect human mixed muscle protein synthesis and muscle phenylalanine kinetics. Am J Physiol Endocrinol Metab. 2008; 295(4):E959-63. PMC: 2575894. DOI: 10.1152/ajpendo.00671.2007. View

15.

Pasiakos S, McClung H, McClung J, Margolis L, Andersen N, Cloutier G . Leucine-enriched essential amino acid supplementation during moderate steady state exercise enhances postexercise muscle protein synthesis. Am J Clin Nutr. 2011; 94(3):809-18. DOI: 10.3945/ajcn.111.017061. View

16.

Phillips S, Tipton K, Aarsland A, Wolf S, Wolfe R . Mixed muscle protein synthesis and breakdown after resistance exercise in humans. Am J Physiol. 1997; 273(1 Pt 1):E99-107. DOI: 10.1152/ajpendo.1997.273.1.E99. View

17.

Ferrando A, Williams B, Stuart C, Lane H, Wolfe R . Oral branched-chain amino acids decrease whole-body proteolysis. JPEN J Parenter Enteral Nutr. 1995; 19(1):47-54. DOI: 10.1177/014860719501900147. View

18.

Witard O, Jackman S, Breen L, Smith K, Selby A, Tipton K . Myofibrillar muscle protein synthesis rates subsequent to a meal in response to increasing doses of whey protein at rest and after resistance exercise. Am J Clin Nutr. 2013; 99(1):86-95. DOI: 10.3945/ajcn.112.055517. View

19.

Devries M, Phillips S . Supplemental protein in support of muscle mass and health: advantage whey. J Food Sci. 2015; 80 Suppl 1:A8-A15. DOI: 10.1111/1750-3841.12802. View

20.

Macnaughton L, Wardle S, Witard O, McGlory C, Hamilton D, Jeromson S . The response of muscle protein synthesis following whole-body resistance exercise is greater following 40 g than 20 g of ingested whey protein. Physiol Rep. 2016; 4(15). PMC: 4985555. DOI: 10.14814/phy2.12893. View