Propofol Protects PC12 Cells from Cobalt Chloride-induced Injury by Mediating MiR-134

Overview

Journal Histol Histopathol

Specialties Anatomy & Histology
Pathology

Date 2021 Jan 7

PMID 33410125

Citations 2

Authors

Hong-Yi Zhou

Fan Jiang

Zhong Cao

Qi-Yun Shen

Yu-Jing Feng

Zhen-Huan Hou

Affiliations

Soon will be listed here.

Abstract

Objective: Propofol (PRO) was reported to exert a neuroprotective effect by decreasing microRNA-134 (miR-134), a brain-specific miRNA, thus, the role of PRO against cobalt chloride (CoCl₂)-induced injury in rat pheochromocytoma cells (PC12) via mediating miR-134 was explored.

Methods: CoCl₂-induced PC12 cells treated with PRO were transfected with or without miR-134 negative control (NC)/ inhibitor/mimic, and the following detections were then performed using cell counting kit-8 (CCK-8), Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) and Hoechst 33258 staining. Autophagy was observed by transmission electron microscope (TEM). Mitochondrial membrane potential (MMP) was detected by Rhodamine-123 (Rh123) staining, and reactive oxygen species (ROS) by dichloro-dihydro-fluorescein diacetate (DCFH-DA) staining. Protein and gene expressions were measured by Western blotting and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), respectively.

Results: PRO reversed the CoCl₂-induced decrease in the PC12 cell viability and increased miR-134 in a dose-dependent manner. CoCl₂ increased LC3II/I ratio and Beclin-1 expression, but decreased p62 expression, which was abolished by PRO. In addition, an increased cell apoptosis rates triggered by CoCl₂ were reduced by PRO with the down-regulations of Bax and Caspase-3 and the up-regulation of Bcl-2. Furthermore, PRO decreased methylenedioxyamphetamine (MDA), nitric oxide (NO) and ROS in CoCl₂-induced PC12 cells accompanying the increase in glutathione peroxidase (GSH-Px) and MMP. The effects of PRO on autophagy, apoptosis and oxidative stress in CoCl₂-induced PC12 cell were reversed by miR-134 mimic.

Conclusion: PRO may mitigate CoCl₂-induced autophagy in PC12 cells with decreased apoptosis and improved oxidative stress via mediating miR-134.

Citing Articles

Research progress of propofol in alleviating cerebral ischemia/reperfusion injury.

Zheng H, Xiao X, Han Y, Wang P, Zang L, Wang L Pharmacol Rep. 2024; 76(5):962-980.

PMID: 38954373 DOI: 10.1007/s43440-024-00620-6.

Crucial role of autophagy in propofol-treated neurological diseases: a comprehensive review.

Yu S, Liao J, Lin X, Luo Y, Lu G Front Cell Neurosci. 2023; 17:1274727.

PMID: 37946715 PMC: 10631783. DOI: 10.3389/fncel.2023.1274727.

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