Environmentally-induced Changes in the Neutral Lipids and Intracellular Vesicles of Saccharomyces Cerevisiae and Kluyveromyces Fragilis

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1977 Aug 26

PMID 334099

Citations 2

Authors

J A Hossack

D M Belk

A H Rose

Affiliations

Soon will be listed here.

Abstract

Saccharomyces cerevisiae, grown aerobically or anaerobically under conditions which induce a requirement for a sterol and an unsaturated fatty acid, synthesized approximately the same amounts of neutral lipid and intracellular low-density vesicles, although the neutral lipids in aerobically-grown cells contained more esterified sterol and less triacylglycerol than those in anaerobically-grown cells. Kluyveromyces fragilis synthesized much less neutral lipid and a smaller quantity of low-density vesicles than S. cerevisiae whether grown at 30 degrees C (generation time 1.1 h) or 20 degrees C (generation time 2.1 h). Both yeasts synthesized highly saturated triacylglycerols, relatively unsaturated phospholipids, and esterified sterols with an intermediate degree of unsaturation irrespective of the conditions under which they were grown. Free sterols in the yeasts were rich in ergosterol and 22(24)-dehydroergosterol, while the esterified sterol fractions were richer in zymosterol.

Citing Articles

Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition.

Thomas D, Rose A Arch Microbiol. 1979; 122(1):49-55.

PMID: 391174 DOI: 10.1007/BF00408045.

Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae.

Thomas D, Hossack J, Rose A Arch Microbiol. 1978; 117(3):239-45.

PMID: 358937 DOI: 10.1007/BF00738541.

References

Fryberg M, Oehlschlager A, Unrau A . Biosynthetic routes to ergosterol in yeast. Biochem Biophys Res Commun. 1972; 48(3):593-7. DOI: 10.1016/0006-291x(72)90389-0. View

Noble A, Duitschaever C . Fatty acid composition of lipids from Saccharomyces fragilis. Lipids. 1973; 8(11):655-7. DOI: 10.1007/BF02533153. View

Bloomfield D, Bloch K . The formation of delta 9-unsaturated fatty acids. J Biol Chem. 1960; 235:337-45. View

Cortat M, Matile P, Wiemken A . Isolation of glucanase-containing vesicles from budding yeast. Arch Mikrobiol. 1972; 82(3):189-205. DOI: 10.1007/BF00412191. View

Clausen M, Christiansen K, Jensen P, Behnke O . Isolation of lipid particles from baker's yeast. FEBS Lett. 1974; 43(2):176-9. DOI: 10.1016/0014-5793(74)80994-4. View

Penman C, Duffus J . Ergosterol is the only sterol in Kluyveromyces fragilis. Antonie Van Leeuwenhoek. 1974; 40(4):529-31. DOI: 10.1007/BF00403816. View

Hunter K, Rose A . Lipid composition of Saccharomyces cerevisiae as influenced by growth temperature. Biochim Biophys Acta. 1972; 260(4):639-53. DOI: 10.1016/0005-2760(72)90013-6. View

Linnane A, Haslam J, Lukins H, Nagley P . The biogenesis of mitochondria in microorganisms. Annu Rev Microbiol. 1972; 26:163-98. DOI: 10.1146/annurev.mi.26.100172.001115. View

Alterthum F, Rose A . Osmotic lysis of sphaeroplasts from Saccharomyces cerevisiae grown anaerobically in media containing different unsaturated fatty acids. J Gen Microbiol. 1973; 77(2):371-82. DOI: 10.1099/00221287-77-2-371. View

10.

Rattray J, Schibeci A, Kidby D . Lipids of yeasts. Bacteriol Rev. 1975; 39(3):197-231. PMC: 413914. DOI: 10.1128/br.39.3.197-231.1975. View

11.

Matile P, Cortat M, Wiemken A, FREY-WYSSLING A . Isolation of glucanase-containing particles from budding Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1971; 68(3):636-40. PMC: 389006. DOI: 10.1073/pnas.68.3.636. View

12.

Steiner M, Lester R . In vitro studies of phospholipid biosynthesis in Saccharomyces cerevisiae. Biochim Biophys Acta. 1972; 260(2):222-43. DOI: 10.1016/0005-2760(72)90035-5. View

13.

Hossack J, Rose A . Fragility of plasma membranes in Saccharomyces cerevisiae enriched with different sterols. J Bacteriol. 1976; 127(1):67-75. PMC: 233034. DOI: 10.1128/jb.127.1.67-75.1976. View

14.

Bauer H, Sigarlakie E, BRACCO U . The lipid globules of Saccharomyces Cerevisiae. A combined chemical and ultrastructural study using ultrathin frozen sections. J Ultrastruct Res. 1975; 51(1):32-9. DOI: 10.1016/s0022-5320(75)80005-0. View

15.

Kates M, HAGEN P . INFLUENCE OF TEMPERATURE ON FATTY ACID COMPOSITION OF PSYCHROPHILIC AND MESOPHILIC SERRATIA SPECIES. Can J Biochem. 1964; 42:481-8. DOI: 10.1139/o64-055. View

16.

Huotari F, Nelson T, Smith F, KIRKWOOD S . Purification of an exo-beta-D-(1 bonded to 3)-glucanase from Basidiomycete species QM 806. J Biol Chem. 1968; 243(5):952-6. View

17.

Nurminen T, Suomalainen H . The lipolytic activities of the isolated cell envelope fracttions of Baker's yeast. Biochem J. 1970; 118(5):759-63. PMC: 1179285. DOI: 10.1042/bj1180759. View

18.

Longley R, Rose A, Knights B . Composition of the protoplast membrane from Saccharomyces cerevisiae. Biochem J. 1968; 108(3):401-12. PMC: 1198825. DOI: 10.1042/bj1080401. View

19.

Sentandreu R, Northcote D . The formation of buds in yeast. J Gen Microbiol. 1969; 55(3):393-8. DOI: 10.1099/00221287-55-3-393. View

20.

Nurminen T, Konttinen K, Suomalainen H . Neutral lipids in the cells and cell envelope fractions of aerobic baker's yeast and anaerobic brewer's yeast. Chem Phys Lipids. 1975; 14(1):15-32. DOI: 10.1016/0009-3084(75)90012-2. View