Wearable Device for Remote Monitoring of Transcutaneous Tissue Oxygenation

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2021 Jan 7

PMID 33408975

Citations 9

Authors

Juan Pedro Cascales

Emmanuel Roussakis

Lilian Witthauer

Avery Goss

Xiaolei Li

Yenyu Chen

Haley L Marks

Conor L Evans

Affiliations

Soon will be listed here.

Abstract

Wearable devices have found widespread applications in recent years as both medical devices as well as consumer electronics for sports and health tracking. A metric of health that is often overlooked in currently available technology is the direct measurement of molecular oxygen in living tissue, a key component in cellular energy production. Here, we report on the development of a wireless wearable prototype for transcutaneous oxygenation monitoring based on quantifying the oxygen-dependent phosphorescence of a metalloporphyrin embedded within a highly breathable oxygen sensing film. The device is completely self-contained, weighs under 30 grams, performs on-board signal analysis, and can communicate with computers or smartphones. The wearable measures tissue oxygenation at the skin surface by detecting the lifetime and intensity of phosphorescence, which undergoes quenching in the presence of oxygen. As well as being insensitive to motion artifacts, it offers robust and reliable measurements even in variable atmospheric conditions related to temperature and humidity. Preliminary testing in a porcine ischemia model shows that the wearable is highly sensitive to changes in tissue oxygenation in the physiological range upon inducing a decrease in limb perfusion.

Citing Articles

Autologous porcine VRAM flap model for VCA research.

Blades C, Dumanian Z, Wang Y, Wang Z, Li B, Washington K Front Transplant. 2024; 3:1504959.

PMID: 39712036 PMC: 11659244. DOI: 10.3389/frtra.2024.1504959.

Continuous oxygen monitoring to enhance ex-vivo organ machine perfusion and reconstructive surgery.

Berkane Y, Cascales J, Roussakis E, Lellouch A, Slade J, Bertheuil N Biosens Bioelectron. 2024; 262:116549.

PMID: 38971037 PMC: 11288283. DOI: 10.1016/j.bios.2024.116549.

Versatile, in-line optical oxygen tension sensors for continuous monitoring during kidney perfusion.

Roussakis E, Cascales J, Yoeli D, Cralley A, Goss A, Wiatrowski A Sens Diagn. 2024; 3(6):1014-1019.

PMID: 38882471 PMC: 11170683. DOI: 10.1039/d3sd00240c.

Recent Technologies for Transcutaneous Oxygen and Carbon Dioxide Monitoring.

Bernasconi S, Angelucci A, De Cesari A, Masotti A, Pandocchi M, Vacca F Diagnostics (Basel). 2024; 14(8).

PMID: 38667431 PMC: 11049249. DOI: 10.3390/diagnostics14080785.

Self-assembled porous polymer films for improved oxygen sensing.

Salaris N, Haigh P, Papakonstantinou I, Tiwari M Sens Actuators B Chem. 2023; 374:132794.

PMID: 37859642 PMC: 10582206. DOI: 10.1016/j.snb.2022.132794.

References

Katayama Y, Fujioka Y, Tsukada K . Development of a Patch-Type Flexible Oxygen Partial Pressure Sensor. IEEE J Transl Eng Health Med. 2020; 8:1400607. PMC: 7333882. DOI: 10.1109/JTEHM.2020.3005477. View

Meier R, Schreml S, Wang X, Landthaler M, Babilas P, Wolfbeis O . Simultaneous photographing of oxygen and pH in vivo using sensor films. Angew Chem Int Ed Engl. 2011; 50(46):10893-6. DOI: 10.1002/anie.201104530. View

Vanderkooi J, Maniara G, Green T, Wilson D . An optical method for measurement of dioxygen concentration based upon quenching of phosphorescence. J Biol Chem. 1987; 262(12):5476-82. View

Clay N, Dent C . Limitations of pulse oximetry to assess limb vascularity. J Bone Joint Surg Br. 1991; 73(2):344. DOI: 10.1302/0301-620X.73B2.2005174. View

Roussakis E, Li Z, Nowell N, Nichols A, Evans C . Bright, "Clickable" Porphyrins for the Visualization of Oxygenation under Ambient Light. Angew Chem Int Ed Engl. 2015; 54(49):14728-31. PMC: 4715754. DOI: 10.1002/anie.201506847. View

Khan Y, Han D, Pierre A, Ting J, Wang X, Lochner C . A flexible organic reflectance oximeter array. Proc Natl Acad Sci U S A. 2018; 115(47):E11015-E11024. PMC: 6255203. DOI: 10.1073/pnas.1813053115. View

Stucker M, Struk P, Hoffmann K, Schulze L, Rochling A, Lubbers D . The transepidermal oxygen flux from the environment is in balance with the capillary oxygen supply. J Invest Dermatol. 2000; 114(3):533-40. DOI: 10.1046/j.1523-1747.2000.00911.x. View

Kranke P, Bennett M, Martyn-St James M, Schnabel A, Debus S, Weibel S . Hyperbaric oxygen therapy for chronic wounds. Cochrane Database Syst Rev. 2015; (6):CD004123. PMC: 7055586. DOI: 10.1002/14651858.CD004123.pub4. View

Babilas P, Liebsch G, Schacht V, Klimant I, Wolfbeis O, Szeimies R . In vivo phosphorescence imaging of pO2 using planar oxygen sensors. Microcirculation. 2005; 12(6):477-87. DOI: 10.1080/10739680591003314. View

10.

Schreml S, Meier R, Kirschbaum M, Kong S, Gehmert S, Felthaus O . Luminescent dual sensors reveal extracellular pH-gradients and hypoxia on chronic wounds that disrupt epidermal repair. Theranostics. 2014; 4(7):721-35. PMC: 4038754. DOI: 10.7150/thno.9052. View

11.

Roussakis E, Cascales J, Marks H, Li X, Grinstaff M, Evans C . Humidity-Insensitive Tissue Oxygen Tension Sensing for Wearable Devices. Photochem Photobiol. 2019; 96(2):373-379. DOI: 10.1111/php.13198. View

12.

RUMSEY W, Vanderkooi J, Wilson D . Imaging of phosphorescence: a novel method for measuring oxygen distribution in perfused tissue. Science. 1988; 241(4873):1649-51. DOI: 10.1126/science.241.4873.1649. View

13.

Lim C, Lee S, Kim J, Kil H, Kim Y, Park J . Wearable, Luminescent Oxygen Sensor for Transcutaneous Oxygen Monitoring. ACS Appl Mater Interfaces. 2018; 10(48):41026-41034. DOI: 10.1021/acsami.8b13276. View

14.

Stucker M, Struk A, Altmeyer P, Herde M, Baumgartl H, Lubbers D . The cutaneous uptake of atmospheric oxygen contributes significantly to the oxygen supply of human dermis and epidermis. J Physiol. 2002; 538(Pt 3):985-94. PMC: 2290093. DOI: 10.1113/jphysiol.2001.013067. View

15.

Vegfors M, Lindberg L, Lennmarken C . The influence of changes in blood flow on the accuracy of pulse oximetry in humans. Acta Anaesthesiol Scand. 1992; 36(4):346-9. DOI: 10.1111/j.1399-6576.1992.tb03479.x. View

16.

Berndt K, Lakowicz J . Electroluminescent lamp-based phase fluorometer and oxygen sensor. Anal Biochem. 1992; 201(2):319-25. DOI: 10.1016/0003-2697(92)90345-8. View

17.

Wang X, Wolfbeis O . Optical methods for sensing and imaging oxygen: materials, spectroscopies and applications. Chem Soc Rev. 2014; 43(10):3666-761. DOI: 10.1039/c4cs00039k. View

18.

Li Z, Roussakis E, Koolen P, Ibrahim A, Kim K, Rose L . Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage. Biomed Opt Express. 2014; 5(11):3748-64. PMC: 4242015. DOI: 10.1364/BOE.5.003748. View

19.

Menzel E, Popovic Z . Picosecond-resolution fluorescence lifetime measuring system with a cw laser and a radio. Rev Sci Instrum. 1978; 49(1):39. DOI: 10.1063/1.1135248. View

20.

Bambot S, Holavanahali R, Lakowicz J, Carter G, Rao G . Phase fluorometric sterilizable optical oxygen sensor. Biotechnol Bioeng. 1994; 43(11):1139-45. DOI: 10.1002/bit.260431119. View