Species in Lower Respiratory Tract of Hospitalized Patients from Shanghai, China: Species Diversity and Emerging Azole Resistance

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2021 Jan 6

PMID 33402838

Citations 9

Authors

Yuan Xu

Min Chen

Junhao Zhu

Bert Gerrits van den Ende

Amanda Juan Chen

Abdullah M S Al-Hatmi

Li Li

Qiangqiang Zhang

Jianping Xu

Wanqing Liao

Yuchong Chen

Affiliations

Soon will be listed here.

Abstract

Purpose: To investigate species diversity and prevalence of antifungal resistance among clinical isolates of spp. in Shanghai, China.

Patients And Methods: In this study, the spp. isolates were analyzed by multilocus sequence typing (MLST) targeting the internal transcribed spacer (ITS) regions, and partial β-tubulin () and calmodulin () genes. The susceptibilities of these isolates to nine antifungal agents were determined according to the protocol in document M38-A3 established by the Clinical and Laboratory Standards Institute (CLSI).

Results: The most common spp. was (58.2%), followed by the complex (23.5%), and complex (15.3%). Isolates belonging to and of the complex and and of the complex were identified. Moreover, several mutations were found in the azole target gene (TR/Y121F/T289A and F46Y, G89G, M172V, N248T and D255E) in azole-resistant isolates of .

Conclusion: The results of our study revealed a diversity of species in the lower respiratory tract of inpatients in Shanghai and approximately 9% of our isolates were resistant to at least one of the triazole antifungals. Formulation of local treatment strategies to combat emerging azole resistance and species diversity in clinically relevant spp. is needed.

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