Systemic and Intestinal Porcine Epidemic Diarrhea Virus-specific Antibody Response and Distribution of Antibody-secreting Cells in Experimentally Infected Conventional Pigs

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2021 Jan 5

PMID 33397461

Citations 14

Authors

Yuto Suda

Ayako Miyazaki

Kohtaro Miyazawa

Tomoyuki Shibahara

Seiichi Ohashi

Affiliations

Soon will be listed here.

Abstract

Porcine epidemic diarrhea (PED) is a coronavirus disease characterized by the rapid spread of severe diarrhea among pigs. PED virus (PEDV) infects and replicates mainly in the epithelial cells of the duodenum, jejunum, ileum and colon. Serum or mucosal IgA antibody levels have been used to predict both vaccine efficacy and the level of protective immunity to enteric infectious diseases in individuals or herds. Details of the B-cell immune response upon PEDV infection, such as the systemic and mucosal PEDV IgA antibody response, the distribution of IgA antibody-secreting cells (ASCs), and their role in virus clearance are not yet clear. In this experimental infection study, we observed similar fluctuations in PEDV IgA antibody levels in serum and intestinal contents of the upper and lower jejunum and ileum, but not fecal samples, over the 4-week experimental course. ASCs that actively secrete PEDV IgA antibody without in vitro stimulation were distributed mainly in the upper jejunum, whereas memory B cells that showed enhanced PEDV IgA antibody production upon in vitro stimulation were observed in mesenteric lymph nodes and the ileum. Our findings will contribute to the development of effective vaccines and diagnostic methods for PEDV.

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References

To T, Ward L, Yuan L, Saif L . Serum and intestinal isotype antibody responses and correlates of protective immunity to human rotavirus in a gnotobiotic pig model of disease. J Gen Virol. 1998; 79 ( Pt 11):2661-72. DOI: 10.1099/0022-1317-79-11-2661. View

de Arriba M, Carvajal A, Pozo J, Rubio P . Mucosal and systemic isotype-specific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus. Vet Immunol Immunopathol. 2002; 85(1-2):85-97. DOI: 10.1016/s0165-2427(01)00417-2. View

Cebra J, Fuhrman J, Griffin P, Rose F, Schweitzer P, Zimmerman D . Changes in specific B cells and the dissemination of the primed state in vivo following antigenic stimulation by different mucosal routes. Ann Allergy. 1984; 53(6 Pt 2):541-9. View

Dastjerdi A, Carr J, Ellis R, Steinbach F, Williamson S . Porcine Epidemic Diarrhea Virus among Farmed Pigs, Ukraine. Emerg Infect Dis. 2015; 21(12):2235-7. PMC: 4672447. DOI: 10.3201/eid2112.150272. View

. Scientific Opinion on porcine epidemic diarrhoea and emerging porcine deltacoronavirus. EFSA J. 2020; 12(10):3877. PMC: 7163704. DOI: 10.2903/j.efsa.2014.3877. View

Van Reeth K, Pensaert M . Prevalence of infections with enzootic respiratory and enteric viruses in feeder pigs entering fattening herds. Vet Rec. 1994; 135(25):594-7. View

Wang D, Fang L, Xiao S . Porcine epidemic diarrhea in China. Virus Res. 2016; 226:7-13. PMC: 7114554. DOI: 10.1016/j.virusres.2016.05.026. View

Masuda T, Murakami S, Takahashi O, Miyazaki A, Ohashi S, Yamasato H . New porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs. Arch Virol. 2015; 160(10):2565-8. PMC: 7087250. DOI: 10.1007/s00705-015-2522-z. View

Saif L, van Cott J, Brim T . Immunity to transmissible gastroenteritis virus and porcine respiratory coronavirus infections in swine. Vet Immunol Immunopathol. 1994; 43(1-3):89-97. PMC: 7119820. DOI: 10.1016/0165-2427(94)90124-4. View

10.

Christiansen D, Earnest-Silveira L, Grubor-Bauk B, Wijesundara D, Boo I, Ramsland P . Pre-clinical evaluation of a quadrivalent HCV VLP vaccine in pigs following microneedle delivery. Sci Rep. 2019; 9(1):9251. PMC: 6592879. DOI: 10.1038/s41598-019-45461-z. View

11.

Lee C . Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus. Virol J. 2015; 12:193. PMC: 4687282. DOI: 10.1186/s12985-015-0421-2. View

12.

Song D, Huang D, Peng Q, Huang T, Chen Y, Zhang T . Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea viruses associated with outbreaks of severe diarrhea in piglets in Jiangxi, China 2013. PLoS One. 2015; 10(3):e0120310. PMC: 4366183. DOI: 10.1371/journal.pone.0120310. View

13.

Ward L, Yuan L, Rosen B, To T, Saif L . Development of mucosal and systemic lymphoproliferative responses and protective immunity to human group A rotaviruses in a gnotobiotic pig model. Clin Diagn Lab Immunol. 1996; 3(3):342-50. PMC: 170344. DOI: 10.1128/cdli.3.3.342-350.1996. View

14.

Langel S, Wang Q, Vlasova A, Saif L . Host Factors Affecting Generation of Immunity Against Porcine Epidemic Diarrhea Virus in Pregnant and Lactating Swine and Passive Protection of Neonates. Pathogens. 2020; 9(2). PMC: 7168134. DOI: 10.3390/pathogens9020130. View

15.

Park S, Kim S, Song D, Park B . Novel porcine epidemic diarrhea virus variant with large genomic deletion, South Korea. Emerg Infect Dis. 2014; 20(12):2089-92. PMC: 4257805. DOI: 10.3201/eid2012.131642. View

16.

Saif L . Mucosal immunity: an overview and studies of enteric and respiratory coronavirus infections in a swine model of enteric disease. Vet Immunol Immunopathol. 1996; 54(1-4):163-9. PMC: 7119851. DOI: 10.1016/s0165-2427(96)05702-9. View

17.

Gimenez-Lirola L, Zhang J, Carrillo-Avila J, Chen Q, Magtoto R, Poonsuk K . Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications. J Clin Microbiol. 2017; 55(5):1426-1436. PMC: 5405260. DOI: 10.1128/JCM.02507-16. View

18.

Kim S, Lee J, Jung J, Kim I, Hyun B, Kim H . Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013. Arch Virol. 2015; 160(4):1055-64. PMC: 7086719. DOI: 10.1007/s00705-015-2353-y. View

19.

de Arriba M, Carvajal A, Pozo J, Rubio P . Isotype-specific antibody-secreting cells in systemic and mucosal associated lymphoid tissues and antibody responses in serum of conventional pigs inoculated with PEDV. Vet Immunol Immunopathol. 2002; 84(1-2):1-16. PMC: 7119739. DOI: 10.1016/s0165-2427(01)00386-5. View

20.

Li W, Li H, Liu Y, Pan Y, Deng F, Song Y . New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis. 2012; 18(8):1350-3. PMC: 3414035. DOI: 10.3201/eid1808.120002. View