Allochthonous and Autochthonous Halothermotolerant Bioanodes From Hypersaline Sediment and Textile Wastewater: A Promising Microbial Electrochemical Process for Energy Recovery Coupled With Real Textile Wastewater Treatment

Overview

Journal Front Bioeng Biotechnol

Date 2021 Jan 4

PMID 33392172

Authors

Refka Askri

Benjamin Erable

Luc Etcheverry

Sirine Saadaoui

Mohamed Neifar

Ameur Cherif

Habib Chouchane

Affiliations

Soon will be listed here.

Abstract

The textile and clothing industry is the first manufacture sector in Tunisia in terms of employment and number of enterprises. It generates large volumes of textile dyeing wastewater (TDWW) containing high concentrations of saline, alkaline, and recalcitrant pollutants that could fuel tenacious and resilient electrochemically active microorganisms in bioanodes of bioelectrochemical systems. In this study, a designed hybrid bacterial halothermotolerant bioanode incorporating indigenous and exogenous bacteria from both hypersaline sediment of Chott El Djerid (HSCE) and TDWW is proposed for simultaneous treatment of real TDWW and anodic current generation under high salinity. For the proposed halothermotolerant bioanodes, electrical current production, chemical oxygen demand (COD) removal efficiency, and bacterial community dynamics were monitored. All the experiments of halothermotolerant bioanode formation have been conducted on 6 cm carbon felt electrodes polarized at -0.1 V/SCE and inoculated with 80% of TDWW and 20% of HSCE for 17 days at 45°C. A reproducible current production of about 12.5 ± 0.2 A/m and a total of 91 ± 3% of COD removal efficiency were experimentally validated. Metagenomic analysis demonstrated significant differences in bacterial diversity mainly at species level between anodic biofilms incorporating allochthonous and autochthonous bacteria and anodic biofilm containing only autochthonous bacteria as a control. Therefore, we concluded that these results provide for the first time a new noteworthy alternative for achieving treatment and recover energy, in the form of a high electric current, from real saline TDWW.

Citing Articles

Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts.

Saidi N, Erable B, Etchevery L, Cherif A, Chouchane H Front Microbiol. 2025; 15():1529841.

PMID: 39834368 PMC: 11743678. DOI: 10.3389/fmicb.2024.1529841.

References

Ben Abdallah M, Karray F, Mhiri N, Mei N, Quemeneur M, Cayol J . Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid. Extremophiles. 2016; 20(2):125-38. DOI: 10.1007/s00792-015-0805-7. View

Grattieri M, Minteer S . Microbial fuel cells in saline and hypersaline environments: Advancements, challenges and future perspectives. Bioelectrochemistry. 2017; 120:127-137. DOI: 10.1016/j.bioelechem.2017.12.004. View

Askri R, Erable B, Neifar M, Etcheverry L, Slaheddine Masmoudi A, Cherif A . Understanding the cumulative effects of salinity, temperature and inoculation size for the design of optimal halothermotolerant bioanodes from hypersaline sediments. Bioelectrochemistry. 2019; 129:179-188. DOI: 10.1016/j.bioelechem.2019.05.015. View

Heidrich E, Dolfing J, Wade M, Sloan W, Quince C, Curtis T . Temperature, inocula and substrate: Contrasting electroactive consortia, diversity and performance in microbial fuel cells. Bioelectrochemistry. 2017; 119:43-50. DOI: 10.1016/j.bioelechem.2017.07.006. View

Ben Abdallah M, Karray F, Kallel N, Armougom F, Mhiri N, Quemeneur M . Abundance and diversity of prokaryotes in ephemeral hypersaline lake Chott El Jerid using Illumina Miseq sequencing, DGGE and qPCR assays. Extremophiles. 2018; 22(5):811-823. DOI: 10.1007/s00792-018-1040-9. View

Rousseau R, Santaella C, Bonnafous A, Achouak W, Godon J, Delia M . Halotolerant bioanodes: The applied potential modulates the electrochemical characteristics, the biofilm structure and the ratio of the two dominant genera. Bioelectrochemistry. 2016; 112:24-32. DOI: 10.1016/j.bioelechem.2016.06.006. View

Vijay A, Arora S, Gupta S, Chhabra M . Halophilic starch degrading bacteria isolated from Sambhar Lake, India, as potential anode catalyst in microbial fuel cell: A promising process for saline water treatment. Bioresour Technol. 2018; 256:391-398. DOI: 10.1016/j.biortech.2018.02.044. View

Shehab N, Ortiz-Medina J, Katuri K, Hari A, Amy G, Logan B . Enrichment of extremophilic exoelectrogens in microbial electrolysis cells using Red Sea brine pools as inocula. Bioresour Technol. 2017; 239:82-86. DOI: 10.1016/j.biortech.2017.04.122. View

Chouchane H, Mahjoubi M, Ettoumi B, Neifar M, Cherif A . A novel thermally stable heteropolysaccharide-based bioflocculant from hydrocarbonoclastic strain Kocuria rosea BU22S and its application in dye removal. Environ Technol. 2017; 39(7):859-872. DOI: 10.1080/09593330.2017.1313886. View

10.

Erable B, Bergel A . First air-tolerant effective stainless steel microbial anode obtained from a natural marine biofilm. Bioresour Technol. 2009; 100(13):3302-7. DOI: 10.1016/j.biortech.2009.02.025. View

11.

Pant D, Van Bogaert G, Diels L, Vanbroekhoven K . A review of the substrates used in microbial fuel cells (MFCs) for sustainable energy production. Bioresour Technol. 2009; 101(6):1533-43. DOI: 10.1016/j.biortech.2009.10.017. View

12.

Holkar C, Jadhav A, Pinjari D, Mahamuni N, Pandit A . A critical review on textile wastewater treatments: Possible approaches. J Environ Manage. 2016; 182:351-366. DOI: 10.1016/j.jenvman.2016.07.090. View

13.

Harnisch F, Freguia S . A basic tutorial on cyclic voltammetry for the investigation of electroactive microbial biofilms. Chem Asian J. 2012; 7(3):466-75. DOI: 10.1002/asia.201100740. View

14.

Berkessa Y, Yan B, Li T, Jegatheesan V, Zhang Y . Treatment of anthraquinone dye textile wastewater using anaerobic dynamic membrane bioreactor: Performance and microbial dynamics. Chemosphere. 2019; 238:124539. DOI: 10.1016/j.chemosphere.2019.124539. View

15.

Jiang M, Ye K, Deng J, Lin J, Ye W, Zhao S . Conventional Ultrafiltration As Effective Strategy for Dye/Salt Fractionation in Textile Wastewater Treatment. Environ Sci Technol. 2018; 52(18):10698-10708. DOI: 10.1021/acs.est.8b02984. View

16.

Ben Mansour H, Dellai A, Ayed Y . Toxicities effects of pharmaceutical, olive mill and textile wastewaters before and after degradation by Pseudomonas putida mt-2. Cancer Cell Int. 2012; 12(1):4. PMC: 3295689. DOI: 10.1186/1475-2867-12-4. View

17.

Yu J, Park Y, Kim B, Lee T . Power densities and microbial communities of brewery wastewater-fed microbial fuel cells according to the initial substrates. Bioprocess Biosyst Eng. 2014; 38(1):85-92. DOI: 10.1007/s00449-014-1246-x. View

18.

Chong P, Erable B, Bergel A . Effect of pore size on the current produced by 3-dimensional porous microbial anodes: A critical review. Bioresour Technol. 2019; 289:121641. DOI: 10.1016/j.biortech.2019.121641. View

19.

Erable B, Roncato M, Achouak W, Bergel A . Sampling natural biofilms: a new route to build efficient microbial anodes. Environ Sci Technol. 2009; 43(9):3194-9. DOI: 10.1021/es803549v. View

20.

Rimboud M, Pocaznoi D, Erable B, Bergel A . Electroanalysis of microbial anodes for bioelectrochemical systems: basics, progress and perspectives. Phys Chem Chem Phys. 2014; 16(31):16349-66. DOI: 10.1039/c4cp01698j. View