Protective Effects of and Against Arsenic Induced Aberrant Methylation and Mitochondrial DNA Damage in Wistar Rat Model

Overview

Journal Toxicol Rep

Date 2021 Jan 4

PMID 33391994

Citations 2

Authors

Amit Datta

Md Jibran Alam

Laila Khaleda

Mohammad Al-Forkan

Affiliations

Soon will be listed here.

Abstract

Millions of people around the world are chronically exposed to Arsenic (As) through food and drinking water. Studies revealed that Arsenic is genotoxic and causes damage to DNA. In this study, we evaluated and for their alleviative properties against Arsenic induced genotoxicity using Wistar Rat model. Arsenic exposed rats were given leaf powder and flower powder as supplementation with normal food. Methylation status of promoter was measured using Methylation Sensitive Restriction Endonuclease PCR (MSRE-PCR) assay and mitochondrial DNA (mtDNA) copy number as well as occurrence of a common deletion in mtDNA in liver and kidney tissue was determined through quantitative realtime PCR (qPCR). Arsenic exposed rats after supplementation showed relatively less severe effects of toxicity evident by significantly higher amount of (0.05) mtDNA copy number and reduced occurrence of deletion containing mtDNA as well as lower levels of methylation in gene promoter. Histopathological analysis revealed less severe histopathological changes of liver and kidney and normal liver and kidney function parameters in supplemented rats. So, the protective properties of and against Arsenic toxicity is evident in molecular level.

Citing Articles

Arsenic-Induced Cardiovascular Diseases and their Correlation with Mitochondrial DNA Copy Number, Deletion, and Telomere Length in Bangladeshi Population.

Khaleda L, Begum S, Apu M, Chowdhury R, Alam M, Datta A Cardiovasc Toxicol. 2023; 24(1):27-40.

PMID: 37971645 DOI: 10.1007/s12012-023-09812-7.

Reduced Peripheral Blood Mitochondrial DNA Copy Number as Identification Biomarker of Suspected Arsenic-Induced Liver Damage.

Wang Q, Ma L, Sun B, Zhang A Biol Trace Elem Res. 2023; 201(11):5083-5097.

PMID: 36720785 DOI: 10.1007/s12011-023-03584-5.

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