Plasmid Protein PORF5 Up-Regulates ZFAS1 to Promote Host Cell Survival Via MAPK/p38 Pathway

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Jan 4

PMID 33391210

Citations 9

Authors

Yating Wen

Hongliang Chen

Fangzhen Luo

Lanhua Zhao

Mingyi Shu

Shengmei Su

Yuqi Zhao

Qiulin Huang

Zhongyu Li

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNAs (lncRNAs) have been demonstrated to play essential roles in many diseases. However, few studies have shown that lncRNAs take part in the pathogenesis of (). Here, we used a lncRNA microarray to detect the global lncRNA expression profiles in HeLa cells transfected with pORF5 plasmid protein, an important virulence factor for . The differentially expressed lncRNAs and mRNAs screened by microarray were selected for validation by quantitative real-time PCR. The up-regulated lncRNA zinc finger antisense 1 (ZFAS1) was presumed to involved in MAPK pathways by bioinformatics analysis. Inhibition of ZFAS1 decreased the apoptotic rate of pORF5 and reduced the infectivity of , and MAPK/p38 pathway was involved in anti-apoptotic effect induced by ZFAS1. Therefore, the present study confirmed that pORF5 up-regulates ZFAS1 to promote host cell survival via MAPK/p38 pathway and influences the infectivity of

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