USP21 Promotes Cell Proliferation by Maintaining the EZH2 Level in Diffuse Large B-cell Lymphoma

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2021 Jan 3

PMID 33389794

Citations 6

Authors

Huixian Ma

Xiangrui Luo

Peng Zhou

Na He

Jun Zhou

Min Liu

Wei Xie

Affiliations

Soon will be listed here.

Abstract

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common category of non-Hodgkin lymphoma (NHL). However, the underlying molecular mechanism of DLBCL remains unclear.

Methods: Real-time PCR and Western blot analysis were performed to assess the expression of ubiquitin-specific peptidase 21 (USP21) or enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2). CCK8 assay and cell death staining were carried out to examine the role of USP21 in cell proliferation and cell death, respectively.

Results: We found that the deubiquitinase USP21 was highly expressed in the DLBCL lymphoid tissue. The expression of USP21 promoted DLBCL cell proliferation, while it had no obvious effect on cell death. In addition, we found that USP21 regulated cell proliferation via cysteine 221, the catalytic site of USP21. Furthermore, we identified that USP21 could stabilize EZH2, a protein required for germinal center formation and lymphoma formation.

Conclusion: The deubiquitinase USP21 promotes cell proliferation by maintaining the EZH2 protein level in DLBCL.

Citing Articles

Ubiquitin-specific peptidases in lymphoma: a path to novel therapeutics.

Samareh Salavatipour M, Tavakoli S, Halimi A, Tavoosi S, Baghsheikhi A, Talebi-Taheri A Front Pharmacol. 2024; 15:1356634.

PMID: 39664521 PMC: 11632177. DOI: 10.3389/fphar.2024.1356634.

Insights Into the Properties, Biological Functions, and Regulation of USP21.

An T, Lu Y, Yan X, Hou J Front Pharmacol. 2022; 13:944089.

PMID: 35846989 PMC: 9279671. DOI: 10.3389/fphar.2022.944089.

Expression and Clinical Value of Eukaryotic Translation Elongation Factor 1A1 (EEF1A1) in Diffuse Large B Cell Lymphoma.

Gong T, Shuang Y Int J Gen Med. 2021; 14:7247-7258.

PMID: 34737619 PMC: 8559353. DOI: 10.2147/IJGM.S324645.

Deubiquitinases in hematological malignancies.

Lei H, Wang J, Hu J, Zhu Q, Wu Y Biomark Res. 2021; 9(1):66.

PMID: 34454635 PMC: 8401176. DOI: 10.1186/s40364-021-00320-w.

Potential role for the tumor suppressor CYLD in brain and notochord development.

Li T, Wang Y, Li D, Zhou J, Zhang B, He X Thorac Cancer. 2021; 12(12):1900-1908.

PMID: 33982884 PMC: 8201528. DOI: 10.1111/1759-7714.13973.

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