Cytotoxic and Antimicrobial Potential of Different Leaves Extracts of Used Traditionally to Treat Diabetes

Overview

Journal Toxicol Rep

Date 2021 Jan 1

PMID 33384939

Citations 9

Authors

Afaf M Weli

Hanady S Al-Saadi

Rouqaya S Al-Fudhaili

Amzad Hossain

Zabida Binti Putit

Mahmood K Jasim

Affiliations

Soon will be listed here.

Abstract

Medicinal plant as herbal medicine is widely used to cure infectious diseases (s) has been studied for its antimicrobial and cytotoxic activities. Different polarity leave extracts were prepared by using hexane, chloroform, ethyl acetate and hydro alcoholic solvents. Agar diffusion method has been used to assess the antibacterial activity against two gram-positive and and two gram-negative and bacterial strains. Cytotoxic activity was carried out against brine shrimp using the nauplii method (BSL). The results so obtained for various experiments demonstrates the total number of extracts produces moderate to strong antibacterial activity against the gram (+ and -). The best activity was discovered within hydro alcoholic upon all concentrations, whereas the inhibition zone exists within the range of 6-11 mm. In addition to that, the cytotoxic activity test confirms that hydro alcoholic extracts the maximum toxicity for values LC (4.68-6.96 μg/ml), where the LC values for all extracts be located within the range of 4.68-6.96 μg/ml. Finally, the plant itself and its derived extracts have been used as a folk medicine to treat serious infectious diseases.

Citing Articles

Seasonal and Altitudinal Effects on Chemical Composition and Rumen Degradability of Blackberry Leaves in Northwestern Italian Alps.

Tassone S, Barbera S, Glorio Patrucco S, Kaihara H, Abid K Animals (Basel). 2025; 15(1.

PMID: 39795053 PMC: 11718945. DOI: 10.3390/ani15010111.

Specific Antimicrobial Activities Revealed by Comparative Evaluation of Selected Gemmotherapy Extracts.

Hejja M, Mihok E, Alaya A, Jolji M, Gyorgy E, Meszaros N Antibiotics (Basel). 2024; 13(2).

PMID: 38391567 PMC: 10885950. DOI: 10.3390/antibiotics13020181.

leaf extract inhibits vascular dementia-induced memory impairment and neuronal loss by attenuating neuroinflammation.

Sung N, Uhm S, Kang H, Lee N, Jeong Y, Kim D Anat Cell Biol. 2023; 56(4):494-507.

PMID: 37743615 PMC: 10714077. DOI: 10.5115/acb.23.195.

Blackberries and Mulberries: Berries with Significant Health-Promoting Properties.

Martins M, Goncalves A, Alves G, Silva L Int J Mol Sci. 2023; 24(15).

PMID: 37569399 PMC: 10418693. DOI: 10.3390/ijms241512024.

Antiviral and Antibacterial Effect of Honey Enriched with spp. as a Functional Food with Enhanced Antioxidant Properties.

Grabek-Lejko D, Milek M, Sidor E, Puchalski C, Dzugan M Molecules. 2022; 27(15).

PMID: 35956811 PMC: 9370118. DOI: 10.3390/molecules27154859.

References

Seremet O, Olaru O, Gutu C, Nitulescu G, Ilie M, Negres S . Toxicity of plant extracts containing pyrrolizidine alkaloids using alternative invertebrate models. Mol Med Rep. 2018; 17(6):7757-7763. PMC: 5983973. DOI: 10.3892/mmr.2018.8795. View

Nguni T, Klaasen J, Fielding B . Acute toxicity studies of the South African medicinal plant . Toxicol Rep. 2018; 5:813-818. PMC: 6098221. DOI: 10.1016/j.toxrep.2018.08.008. View

Wada L, Ou B . Antioxidant activity and phenolic content of Oregon caneberries. J Agric Food Chem. 2002; 50(12):3495-500. DOI: 10.1021/jf011405l. View

Olaru O, Venables L, van de Venter M, Nitulescu G, Margina D, Spandidos D . Anticancer potential of selected Adans species. Oncol Lett. 2015; 10(3):1323-1332. PMC: 4533735. DOI: 10.3892/ol.2015.3453. View

Marquina M, Corao G, Araujo L, Buitrago D, Sosa M . Hyaluronidase inhibitory activity from the polyphenols in the fruit of blackberry (Rubus fruticosus B.). Fitoterapia. 2002; 73(7-8):727-9. DOI: 10.1016/s0367-326x(02)00222-8. View

Jakobsdottir G, Blanco N, Xu J, Ahrne S, Molin G, Sterner O . Formation of short-chain Fatty acids, excretion of anthocyanins, and microbial diversity in rats fed blackcurrants, blackberries, and raspberries. J Nutr Metab. 2013; 2013:202534. PMC: 3707259. DOI: 10.1155/2013/202534. View

Al-Mqbali L, Hossain M . Cytotoxic and antimicrobial potential of different varieties of ripe banana used traditionally to treat ulcers. Toxicol Rep. 2019; 6:1086-1090. PMC: 6807309. DOI: 10.1016/j.toxrep.2019.10.003. View

Patel A, Rojas-Vera J, Dacke C . Therapeutic constituents and actions of Rubus species. Curr Med Chem. 2004; 11(11):1501-12. DOI: 10.2174/0929867043365143. View

Zia-Ul-Haq M, Riaz M, De Feo V, Jaafar H, Moga M . Rubus fruticosus L.: constituents, biological activities and health related uses. Molecules. 2014; 19(8):10998-1029. PMC: 6271759. DOI: 10.3390/molecules190810998. View

10.

Kaume L, Howard L, Devareddy L . The blackberry fruit: a review on its composition and chemistry, metabolism and bioavailability, and health benefits. J Agric Food Chem. 2011; 60(23):5716-27. DOI: 10.1021/jf203318p. View

11.

Bailey C, Day C . Traditional plant medicines as treatments for diabetes. Diabetes Care. 1989; 12(8):553-64. DOI: 10.2337/diacare.12.8.553. View

12.

Ali N, Aleem U, Ali Shah S, Shah I, Junaid M, Ahmed G . Acute toxicity, brine shrimp cytotoxicity, anthelmintic and relaxant potentials of fruits of Rubus fruticosus Agg. BMC Complement Altern Med. 2013; 13:138. PMC: 3688410. DOI: 10.1186/1472-6882-13-138. View

13.

Aymoto Hassimotto N, da Silva Pinto M, Lajolo F . Antioxidant status in humans after consumption of blackberry (Rubus fruticosus L.) juices with and without defatted milk. J Agric Food Chem. 2008; 56(24):11727-33. DOI: 10.1021/jf8026149. View

14.

Harwig J, Scott P . Brine shrimp (Artemia salina L.) larvae as a screening system for fungal toxins. Appl Microbiol. 1971; 21(6):1011-6. PMC: 377334. DOI: 10.1128/am.21.6.1011-1016.1971. View

15.

Verma R, Gangrade T, Punasiya R, Ghulaxe C . Rubus fruticosus (blackberry) use as an herbal medicine. Pharmacogn Rev. 2014; 8(16):101-4. PMC: 4127818. DOI: 10.4103/0973-7847.134239. View

16.

Kanegusuku M, Benassi J, Pedrosa R, Yunes R, Filho V, Maia A . Cytotoxic, hypoglycemic activity and phytochemical analysis of Rubus imperialis (Rosaceae). Z Naturforsch C J Biosci. 2002; 57(3-4):272-6. DOI: 10.1515/znc-2002-3-412. View

17.

Sellappan S, Akoh C, Krewer G . Phenolic compounds and antioxidant capacity of Georgia-grown blueberries and blackberries. J Agric Food Chem. 2002; 50(8):2432-8. DOI: 10.1021/jf011097r. View

18.

Seremet O, Barbuceanu F, Ionica F, Margina D, Gutu C, Olaru O . Oral toxicity study of certain plant extracts containing pyrrolizidine alkaloids. Rom J Morphol Embryol. 2016; 57(3):1017-1023. View