Dental Pulp Stem Cells from Human Teeth with Deep Caries Displayed an Enhanced Angiogenesis Potential

Overview

Journal J Dent Sci

Specialty Dentistry

Date 2021 Jan 1

PMID 33384815

Citations 8

Authors

Yan Chen

Xinzhu Li

Jingyi Wu

Wanyu Lu

Wenan Xu

Buling Wu

Affiliations

Soon will be listed here.

Abstract

Background/purpose: Dental pulp stem cells can be isolated from human teeth with deep caries (cDPSCs), but their biological characteristics are still unclear. The aim of this study was to investigate the angiogenic potential of cDPSCs and compare them to dental pulp stem cells from human normal teeth (nDPSCs).

Materials And Methods: Cells were isolated from human pulp tissue of normal and infected teeth with deep caries. Basic mesenchymal stem cell (MSC) characterization was conducted. Colony forming units and proliferation ability were evaluated in nDPSCs and cDPSCs. Expression of VEGF in both tissues and cells was examined by immunohistochemical staining. After stimulating nDPSCs and cDPSCs with an angiogenic medium, angiogenic markers were evaluated by qRT-PCR and western blotting. Finally, tube formation assays were used to evaluate the angiogenesis potential of both cell populations.

Results: Both nDPSCs and cDPSCs possessed typical MSC characteristics. cDPSCs had enhanced colony formation and proliferation capacities than nDPSCs did. The expression of VEGF was higher in pulp tissue from teeth with deep caries and cDPSCs than in normal tissue and nDPSCs. When both cell types were grown under angiogenic conditions, cDPSCs expressed a higher level of angiogenic markers and showed a stronger angiogenesis potential than nDPSCs did.

Conclusion: cDPSCs maintained MSC traits and presented a higher angiogenesis potential than nDPSCs.

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