Cybrid Model Supports Mitochondrial Genetic Effect on Pig Litter Size

Overview

Journal Front Genet

Date 2021 Jan 1

PMID 33384712

Citations 3

Authors

Hao Liu

Jikun Wang

Dan Wang

Minghua Kong

Chao Ning

Xing Zhang

Jinlong Xiao

Xin Zhang

Jianfeng Liu

Xingbo Zhao

Affiliations

Soon will be listed here.

Abstract

In pigs, mitochondrial DNA (mtDNA) polymorphism and the correlation to reproductive performance across breeds and individuals have been largely reported, however, experimental proof has never been provided. In this study, we analyzed 807 sows for correlation of total number born (TNB) and mitotype, which presented the maximum of 1.73 piglets for mtDNA contribution. Cybrid models representing different mitotypes were generated for identification of the mtDNA effect. Results indicated significant differences on cellular and molecular characteristics among cybrids, including energy metabolic traits, mtDNA copy numbers and transcriptions, mRNA and protein expressions on mitochondrial biogenesis genes and reproduction-related genes. Referring to mitotypes, the cybrids with prolific mitotypes presented significantly higher oxygen consumption rate (OCR) productions, mtDNA transcriptions and copy numbers than those with common mitotypes, while both mRNA and protein expressions of , , , , and in prolific cybrids were significantly higher than those with common mitotypes. Cybrid models reflected the mtDNA effect on pig litter size, suggesting the potential application of mtDNA polymorphism in pig selection and breeding practices.

Citing Articles

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References

Lee W, Johnson J, Gough D, Donoghue J, Cagnone G, Vaghjiani V . Mitochondrial DNA copy number is regulated by DNA methylation and demethylation of POLGA in stem and cancer cells and their differentiated progeny. Cell Death Dis. 2015; 6:e1664. PMC: 4669800. DOI: 10.1038/cddis.2015.34. View

van der Bliek A, Sedensky M, Morgan P . Cell Biology of the Mitochondrion. Genetics. 2017; 207(3):843-871. PMC: 5676242. DOI: 10.1534/genetics.117.300262. View

Choi Y, Jeong J, Min H, Jung H, Hwang D, Lee S . Shot-gun proteomic analysis of mitochondrial D-loop DNA binding proteins: identification of mitochondrial histones. Mol Biosyst. 2011; 7(5):1523-36. DOI: 10.1039/c0mb00277a. View

Wang X, Lu X, Zhu R, Zhang K, Li S, Chen Z . Betulinic Acid Induces Apoptosis in Differentiated PC12 Cells Via ROS-Mediated Mitochondrial Pathway. Neurochem Res. 2017; 42(4):1130-1140. DOI: 10.1007/s11064-016-2147-y. View

Kaniak-Golik A, Skoneczna A . Mitochondria-nucleus network for genome stability. Free Radic Biol Med. 2015; 82:73-104. DOI: 10.1016/j.freeradbiomed.2015.01.013. View

Goffart S, Wiesner R . Regulation and co-ordination of nuclear gene expression during mitochondrial biogenesis. Exp Physiol. 2003; 88(1):33-40. DOI: 10.1113/eph8802500. View

Wai T, Ao A, Zhang X, Cyr D, Dufort D, Shoubridge E . The role of mitochondrial DNA copy number in mammalian fertility. Biol Reprod. 2010; 83(1):52-62. PMC: 2888963. DOI: 10.1095/biolreprod.109.080887. View

Fan W, He N, Lin C, Wei Z, Hah N, Waizenegger W . ERRγ Promotes Angiogenesis, Mitochondrial Biogenesis, and Oxidative Remodeling in PGC1α/β-Deficient Muscle. Cell Rep. 2018; 22(10):2521-2529. PMC: 5860878. DOI: 10.1016/j.celrep.2018.02.047. View

St John J, Tsai T . The association of mitochondrial DNA haplotypes and phenotypic traits in pigs. BMC Genet. 2018; 19(1):41. PMC: 6035439. DOI: 10.1186/s12863-018-0629-4. View

10.

Xiang H, Gao J, Yu B, Zhou H, Cai D, Zhang Y . Early Holocene chicken domestication in northern China. Proc Natl Acad Sci U S A. 2014; 111(49):17564-9. PMC: 4267363. DOI: 10.1073/pnas.1411882111. View

11.

Wallace D . Mitochondria and cancer. Nat Rev Cancer. 2012; 12(10):685-98. PMC: 4371788. DOI: 10.1038/nrc3365. View

12.

Reicher S, Seroussi E, Weller J, Rosov A, Gootwine E . Ovine mitochondrial DNA sequence variation and its association with production and reproduction traits within an Afec-Assaf flock. J Anim Sci. 2012; 90(7):2084-91. DOI: 10.2527/jas.2011-4673. View

13.

Almeida Santos T, El Shourbagy S, St John J . Mitochondrial content reflects oocyte variability and fertilization outcome. Fertil Steril. 2006; 85(3):584-91. DOI: 10.1016/j.fertnstert.2005.09.017. View

14.

Wallace D . Mitochondrial DNA sequence variation in human evolution and disease. Proc Natl Acad Sci U S A. 1994; 91(19):8739-46. PMC: 44682. DOI: 10.1073/pnas.91.19.8739. View

15.

Tsai T, Rajasekar S, St John J . The relationship between mitochondrial DNA haplotype and the reproductive capacity of domestic pigs (Sus scrofa domesticus). BMC Genet. 2016; 17(1):67. PMC: 4870755. DOI: 10.1186/s12863-016-0375-4. View

16.

Moraes C . What regulates mitochondrial DNA copy number in animal cells?. Trends Genet. 2001; 17(4):199-205. DOI: 10.1016/s0168-9525(01)02238-7. View

17.

Laubenthal L, Hoelker M, Frahm J, Danicke S, Gerlach K, Sudekum K . Mitochondrial DNA copy number and biogenesis in different tissues of early- and late-lactating dairy cows. J Dairy Sci. 2015; 99(2):1571-1583. DOI: 10.3168/jds.2015-9847. View

18.

Boengler K, Kosiol M, Mayr M, Schulz R, Rohrbach S . Mitochondria and ageing: role in heart, skeletal muscle and adipose tissue. J Cachexia Sarcopenia Muscle. 2017; 8(3):349-369. PMC: 5476857. DOI: 10.1002/jcsm.12178. View

19.

Li L, Xiao L, Hou Y, He Q, Zhu J, Li Y . Sestrin2 Silencing Exacerbates Cerebral Ischemia/Reperfusion Injury by Decreasing Mitochondrial Biogenesis through the AMPK/PGC-1α Pathway in Rats. Sci Rep. 2016; 6:30272. PMC: 4958997. DOI: 10.1038/srep30272. View

20.

King A, Jiang Z, Gibson J, Haley C, Archibald A . Mapping quantitative trait loci affecting female reproductive traits on porcine chromosome 8. Biol Reprod. 2003; 68(6):2172-9. DOI: 10.1095/biolreprod.102.012955. View