Genetic Factors Associated With Nodulation and Nitrogen Derived From Atmosphere in a Middle American Common Bean Panel

Overview

Journal Front Plant Sci

Date 2021 Jan 1

PMID 33384700

Citations 7

Authors

Atena Oladzad

Abiezer Gonzalez

Raul Macchiavelli

Consuelo Estevez de Jensen

James Beaver

Tim Porch

Phillip McClean

Affiliations

Soon will be listed here.

Abstract

Among grain legume crops, common beans ( L.) are considered to have poor biological nitrogen (N) fixation (BNF) capabilities although variation in N fixing capabilities exists within the species. The availability of genetic panel varying in BNF capacity and a large-scale single nucleotide polymorphism (SNP) data set for common bean provided an opportunity to discover genetic factors associated with N fixation among genotypes in the Middle American gene pool. Using nodulation and percentage of N-derived from atmosphere (%NDFA) data collected from field trials, at least 11 genotypes with higher levels of BNF capacity were identified. Genome-wide association studies (GWASs) detected both major and minor effects that control these traits. A major nodulation interval at Pv06:28.0-28.27 Mbp was discovered. In this interval, the peak SNP was located within a small GTPase that positively regulates cellular polarity and growth of root hair tips. Located 20 kb upstream of this peak SNP is an auxin-responsive factor AUX/indole acetic auxin (IAA)-related gene involved in auxin transportation during root nodulation. For %NDFA, nitrate (NO ) transporters, and (Pv02:8.64), squamosa promoter binding transcriptome factor (Pv08:28.42), and multi-antimicrobial extrusion protein (MATE) efflux family protein (Pv06:10.91) were identified as candidate genes. Three additional QTLs were identified on chromosomes Pv03:5.24, Pv09:25.89, and Pv11: 32.89 Mbp. These key candidate genes from both traits were integrated with previous results on N fixation to describe a BNF pathway.

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