Arsenic Exposure and Cancer-Related Proteins in Urine of Indigenous Bolivian Women

Overview

Journal Front Public Health

Specialty Public Health

Date 2020 Dec 31

PMID 33381488

Citations 7

Authors

Jessica De Loma

Anda R Gliga

Michael Levi

Franz Ascui

Jacques Gardon

Noemi Tirado

Karin Broberg

Affiliations

Soon will be listed here.

Abstract

Indigenous people living in the Bolivian Andes are exposed through their drinking water to inorganic arsenic, a potent carcinogen. However, the health consequences of arsenic exposure in this region are unknown. The aim of this study was to evaluate associations between arsenic exposure and changes in cancer-related proteins in indigenous women ( = 176) from communities around the Andean Lake Poopó, Bolivia. Arsenic exposure was assessed in whole blood (B-As) and urine (as the sum of arsenic metabolites, U-As) by inductively coupled plasma-mass spectrometry (ICP-MS). Cancer-related proteins ( = 92) were measured in urine using the proximity extension assay. The median B-As concentration was 2.1 (range 0.60-9.1) ng/g, and U-As concentration was 67 (12-399) μg/L. Using linear regression models adjusted for age, urinary osmolality, and urinary leukocytes, we identified associations between B-As and four putative cancer-related proteins: FASLG, SEZ6L, LYPD3, and TFPI2. Increasing B-As concentrations were associated with lower protein expression of SEZ6L, LYPD3, and TFPI2, and with higher expression of FASLG in urine (no association was statistically significant after correcting for multiple comparisons). The associations were similar across groups with different arsenic metabolism efficiency, a susceptibility factor for arsenic toxicity. In conclusion, arsenic exposure in this region was associated with changes in the expression of some cancer-related proteins in urine. Future research is warranted to understand if these proteins could serve as valid biomarkers for arsenic-related toxicity.

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References

Chadha V, Garg U, Alon U . Measurement of urinary concentration: a critical appraisal of methodologies. Pediatr Nephrol. 2001; 16(4):374-82. DOI: 10.1007/s004670000551. View

Kuo C, Moon K, Wang S, Silbergeld E, Navas-Acien A . The Association of Arsenic Metabolism with Cancer, Cardiovascular Disease, and Diabetes: A Systematic Review of the Epidemiological Evidence. Environ Health Perspect. 2017; 125(8):087001. PMC: 5880251. DOI: 10.1289/EHP577. View

Hu P, Huang Y, Gao Y, Yan H, Li X, Zhang J . Elevated Expression of Is Associated with Lung Adenocarcinoma Carcinogenesis and Poor Prognosis. DNA Cell Biol. 2020; 39(4):522-532. DOI: 10.1089/dna.2019.5116. View

Kadam C, Abhang S . Apoptosis Markers in Breast Cancer Therapy. Adv Clin Chem. 2016; 74:143-93. DOI: 10.1016/bs.acc.2015.12.003. View

Zeng Y, Weng G, Fan J, Li Z, Wu J, Li Y . Curcumin reduces the expression of survivin, leading to enhancement of arsenic trioxide-induced apoptosis in myelodysplastic syndrome and leukemia stem-like cells. Oncol Rep. 2016; 36(3):1233-42. PMC: 5001835. DOI: 10.3892/or.2016.4944. View

Gorlov I, Meyer P, Liloglou T, Myles J, Boettger M, Cassidy A . Seizure 6-like (SEZ6L) gene and risk for lung cancer. Cancer Res. 2007; 67(17):8406-11. DOI: 10.1158/0008-5472.CAN-06-4784. View

Vahter M . Mechanisms of arsenic biotransformation. Toxicology. 2002; 181-182:211-7. DOI: 10.1016/s0300-483x(02)00285-8. View

Zhai R, Su S, Lu X, Liao R, Ge X, He M . Proteomic profiling in the sera of workers occupationally exposed to arsenic and lead: identification of potential biomarkers. Biometals. 2006; 18(6):603-13. DOI: 10.1007/s10534-005-3001-x. View

Zhang Q, Zhang Y, Wang S, Wang N, Jiang W, Ji Y . Reduced expression of tissue factor pathway inhibitor-2 contributes to apoptosis and angiogenesis in cervical cancer. J Exp Clin Cancer Res. 2012; 31:1. PMC: 3314549. DOI: 10.1186/1756-9966-31-1. View

10.

Salgado-Bustamante M, Ortiz-Perez M, Calderon-Aranda E, Estrada-Capetillo L, Nino-Moreno P, Gonzalez-Amaro R . Pattern of expression of apoptosis and inflammatory genes in humans exposed to arsenic and/or fluoride. Sci Total Environ. 2009; 408(4):760-7. DOI: 10.1016/j.scitotenv.2009.11.016. View

11.

Lorenzo Bermejo J, Boekstegers F, Gonzalez Silos R, Marcelain K, Baez Benavides P, Ponce C . Subtypes of Native American ancestry and leading causes of death: Mapuche ancestry-specific associations with gallbladder cancer risk in Chile. PLoS Genet. 2017; 13(5):e1006756. PMC: 5444600. DOI: 10.1371/journal.pgen.1006756. View

12.

Assarsson E, Lundberg M, Holmquist G, Bjorkesten J, Thorsen S, Ekman D . Homogenous 96-plex PEA immunoassay exhibiting high sensitivity, specificity, and excellent scalability. PLoS One. 2014; 9(4):e95192. PMC: 3995906. DOI: 10.1371/journal.pone.0095192. View

13.

Li H, Engstrom K, Vahter M, Broberg K . Arsenic exposure through drinking water is associated with longer telomeres in peripheral blood. Chem Res Toxicol. 2012; 25(11):2333-9. PMC: 3501147. DOI: 10.1021/tx300222t. View

14.

Kim S, Han Y, Song J, Lee C, Kang K, Yi J . Aberrantly hypermethylated tumor suppressor genes were identified in oral squamous cell carcinoma (OSCC). Clin Epigenetics. 2019; 11(1):116. PMC: 6689875. DOI: 10.1186/s13148-019-0715-0. View

15.

Engstrom K, Wojdacz T, Marabita F, Ewels P, Kaller M, Vezzi F . Transcriptomics and methylomics of CD4-positive T cells in arsenic-exposed women. Arch Toxicol. 2016; 91(5):2067-2078. PMC: 5399044. DOI: 10.1007/s00204-016-1879-4. View

16.

Clark S, Melki J . DNA methylation and gene silencing in cancer: which is the guilty party?. Oncogene. 2002; 21(35):5380-7. DOI: 10.1038/sj.onc.1205598. View

17.

Fellstrom B, Helmersson-Karlqvist J, Lind L, Soveri I, Wu P, Thulin M . Associations Between Apolipoprotein A1, High-Density Lipoprotein Cholesterol, and Urinary Cytokine Levels in Elderly Males and Females. J Interferon Cytokine Res. 2019; 40(2):71-74. DOI: 10.1089/jir.2019.0074. View

18.

Harris S, Cox S, Bell S, Marioni R, Prins B, Pattie A . Neurology-related protein biomarkers are associated with cognitive ability and brain volume in older age. Nat Commun. 2020; 11(1):800. PMC: 7010796. DOI: 10.1038/s41467-019-14161-7. View

19.

Sun F, Fan Y, Zhao J, Zhang F, Gao S, Zhao Z . Detection of TFPI2 methylation in the serum of hepatocellular carcinoma patients. Dig Dis Sci. 2012; 58(4):1010-5. DOI: 10.1007/s10620-012-2462-3. View

20.

Levi M, Hjelm C, Harari F, Vahter M . ICP-MS measurement of toxic and essential elements in human breast milk. A comparison of alkali dilution and acid digestion sample preparation methods. Clin Biochem. 2017; 53:81-87. DOI: 10.1016/j.clinbiochem.2017.12.003. View