Identification of Allelic Variation in Drought Responsive Dehydrin Gene Based on Sequence Similarity in Chickpea ( L.)

Overview

Journal Front Genet

Date 2020 Dec 31

PMID 33381148

Citations 5

Authors

Tapan Kumar

Neha Tiwari

Chellapilla Bharadwaj

Ashutosh Sarker

Sneha Priya Reddy Pappula

Sarvjeet Singh

Mohar Singh

Affiliations

Soon will be listed here.

Abstract

Chickpea ( L.) is an economically important food legume grown in arid and semi-arid regions of the world. Chickpea is cultivated mainly in the rainfed, residual moisture, and restricted irrigation condition. The crop is always prone to drought stress which is resulting in flower drop, unfilled pods, and is a major yield reducer in many parts of the world. The present study elucidates the association between candidate gene and morpho-physiological traits for the screening of drought tolerance in chickpea. Abiotic stress-responsive gene Dehydrin () was identified in some of the chickpea genotypes based on the sequence similarity approach to play a major role in drought tolerance. Analysis of variance revealed a significant effect of drought on relative water content, membrane stability index, plant height, and yield traits. The genotypes Pusa1103, Pusa362, and ICC4958 were found most promising genotypes for drought tolerance as they maintained the higher value of osmotic regulations and yield characters. The results were further supported by a sequence similarity approach for the dehydrin gene when analyzed for the presence of single nucleotide polymorphisms (SNPs) and indels. Homozygous indels and single nucleotide polymorphisms were found after the sequencing in some of the selected genotypes.

Citing Articles

Molecular Breeding and Drought Tolerance in Chickpea.

Asati R, Tripathi M, Tiwari S, Yadav R, Tripathi N Life (Basel). 2022; 12(11).

PMID: 36430981 PMC: 9698494. DOI: 10.3390/life12111846.

A comprehensive analysis of Trehalose-6-phosphate synthase (TPS) gene for salinity tolerance in chickpea (Cicer arietinum L.).

Kumar T, Tiwari N, Bharadwaj C, Roorkiwal M, Reddy S, Patil B Sci Rep. 2022; 12(1):16315.

PMID: 36175531 PMC: 9523030. DOI: 10.1038/s41598-022-20771-x.

Unraveling Origin, History, Genetics, and Strategies for Accelerated Domestication and Diversification of Food Legumes.

Ambika , Aski M, Gayacharan , Hamwieh A, Talukdar A, Gupta S Front Genet. 2022; 13:932430.

PMID: 35979429 PMC: 9376740. DOI: 10.3389/fgene.2022.932430.

Identification of drought tolerant Chickpea genotypes through multi trait stability index.

Hussain T, Akram Z, Shabbir G, Manaf A, Ahmed M Saudi J Biol Sci. 2021; 28(12):6818-6828.

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Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination.

Kundratova K, Bartas M, Pecinka P, Hejna O, Rychla A, curn V Plants (Basel). 2021; 10(9).

PMID: 34579414 PMC: 8465278. DOI: 10.3390/plants10091878.

References

Bharadwaj C, Srivastava R, Chauhan S, Satyavathi C, Kumar J, Faruqui A . Molecular diversity and phylogeny in geographical collection of chickpea (Cicer sp.) accessions. J Genet. 2012; 90(3):e94-100. View

Devasirvatham V, Gaur P, Mallikarjuna N, Tokachichu R, Trethowan R, Tan D . Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments. Funct Plant Biol. 2020; 39(12):1009-1018. DOI: 10.1071/FP12033. View

Allagulova C, Gimalov F, Shakirova F, Vakhitov V . The plant dehydrins: structure and putative functions. Biochemistry (Mosc). 2003; 68(9):945-51. DOI: 10.1023/a:1026077825584. View

Lata C, Prasad M . Role of DREBs in regulation of abiotic stress responses in plants. J Exp Bot. 2011; 62(14):4731-48. DOI: 10.1093/jxb/err210. View

Puhakainen T, Hess M, Makela P, Svensson J, Heino P, Palva E . Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis. Plant Mol Biol. 2004; 54(5):743-53. DOI: 10.1023/B:PLAN.0000040903.66496.a4. View

Varshney R, Song C, Saxena R, Azam S, Yu S, Sharpe A . Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nat Biotechnol. 2013; 31(3):240-6. DOI: 10.1038/nbt.2491. View

Ray S, Satya P . Next generation sequencing technologies for next generation plant breeding. Front Plant Sci. 2014; 5:367. PMC: 4115663. DOI: 10.3389/fpls.2014.00367. View

Jain D, Chattopadhyay D . Analysis of gene expression in response to water deficit of chickpea (Cicer arietinum L.) varieties differing in drought tolerance. BMC Plant Biol. 2010; 10:24. PMC: 2831037. DOI: 10.1186/1471-2229-10-24. View

Maqbool B, Zhong H, El-Maghraby Y, Ahmad A, Chai B, Wang W . Competence of oat ( Avena sativa L.) shoot apical meristems for integrative transformation, inherited expression, and osmotic tolerance of transgenic lines containing hva1. Theor Appl Genet. 2003; 105(2-3):201-208. DOI: 10.1007/s00122-002-0984-3. View

10.

Deshmukh R, Sonah H, Patil G, Chen W, Prince S, Mutava R . Integrating omic approaches for abiotic stress tolerance in soybean. Front Plant Sci. 2014; 5:244. PMC: 4042060. DOI: 10.3389/fpls.2014.00244. View

11.

Wise M, Tunnacliffe A . POPP the question: what do LEA proteins do?. Trends Plant Sci. 2004; 9(1):13-7. DOI: 10.1016/j.tplants.2003.10.012. View

12.

Shah T, Imran M, Atta B, Ashraf M, Hameed A, Waqar I . Selection and screening of drought tolerant high yielding chickpea genotypes based on physio-biochemical indices and multi-environmental yield trials. BMC Plant Biol. 2020; 20(1):171. PMC: 7164285. DOI: 10.1186/s12870-020-02381-9. View

13.

Kim H, Lee J, Kim J, Kim C, Jun S, Hong Y . Molecular and functional characterization of CaLEA6, the gene for a hydrophobic LEA protein from Capsicum annuum. Gene. 2005; 344:115-23. DOI: 10.1016/j.gene.2004.09.012. View

14.

da Silva J, Naylor A, Kramer P . Some ultrastructural and enzymatic effects of water stress in cotton (gossypium hirsutum L.) leaves. Proc Natl Acad Sci U S A. 1974; 71(8):3243-7. PMC: 388660. DOI: 10.1073/pnas.71.8.3243. View

15.

Perez-de-Castro A, Vilanova S, Canizares J, Pascual L, Blanca J, Diez M . Application of genomic tools in plant breeding. Curr Genomics. 2012; 13(3):179-95. PMC: 3382273. DOI: 10.2174/138920212800543084. View

16.

Vasudevan K, Gruissem W, Bhullar N . Identification of novel alleles of the rice blast resistance gene Pi54. Sci Rep. 2015; 5:15678. PMC: 4620502. DOI: 10.1038/srep15678. View

17.

Kumari S, Sabharwal V, Kushwaha H, Sopory S, Singla-Pareek S, Pareek A . Transcriptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L. Funct Integr Genomics. 2008; 9(1):109-23. DOI: 10.1007/s10142-008-0088-5. View

18.

Puhakainen T, Li C, Boije-Malm M, Kangasjarvi J, Heino P, Palva E . Short-day potentiation of low temperature-induced gene expression of a C-repeat-binding factor-controlled gene during cold acclimation in silver birch. Plant Physiol. 2004; 136(4):4299-307. PMC: 535859. DOI: 10.1104/pp.104.047258. View

19.

Moons A, De Keyser A, Van Montagu M . A group 3 LEA cDNA of rice, responsive to abscisic acid, but not to jasmonic acid, shows variety-specific differences in salt stress response. Gene. 1997; 191(2):197-204. DOI: 10.1016/s0378-1119(97)00059-0. View

20.

Upadhyaya H, Kashiwagi J, Varshney R, Gaur P, Saxena K, Krishnamurthy L . Phenotyping chickpeas and pigeonpeas for adaptation to drought. Front Physiol. 2012; 3:179. PMC: 3365634. DOI: 10.3389/fphys.2012.00179. View