Establishment of a Long-Term Co-culture Assay For Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2020 Dec 30

PMID 33377055

Authors

Sayantani Sinha

Sayan Chakraborty

Amitava Sengupta

Affiliations

Soon will be listed here.

Abstract

We describe a protocol for a long-term co-culture assay to study the contribution of mesenchymal stromal cells (MSCs) in regulating hematopoietic stem/progenitor cell (HSPC) activity. In addition, we describe the use of a clonogenic assay to determine myelo-erythroid differentiation. This long-term culture-initiating cell assay can be used for qualitative analysis of MSCs capable of supporting hematopoiesis and may also be used as a proxy readout to study HSPC repopulation. For complete details on the use and execution of this protocol, please refer to Sinha et al. (2020).

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