Comparison of the Morphology and Developmental Potential of Oocytes Obtained from Prepubertal and Adult Domestic and Wild Cats

Overview

Journal Animals (Basel)

Date 2020 Dec 30

PMID 33374370

Citations 4

Authors

Joanna Kochan

Agnieszka Nowak

Wieslawa Mlodawska

Sylwia Prochowska

Agnieszka Partyka

Jozef Skotnicki

Wojciech Nizanski

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to compare the morphology and developmental potential of oocytes obtained from adult and prepubertal domestic cats () and wild cats (, , , ). The average number of oocytes obtained from an adult domestic cat was 23 ± 11, which was significantly lower than from kittens (43 ± 29). A similar number of oocytes was derived from adult Pallas's cats (28 ± 8), and serval (30). The lowest number of oocytes was collected from the lynx (5 ± 3). No oocytes were obtained from newborn Amur tiger while in the case of older domestic and Pallas's cat and lynx kittens (1-3 months) 43, 48 and 41 oocytes were collected, respectively. Significant differences ( < 0.001) were observed between the number of oocytes with dark cytoplasm from adult and prepubertal animals of all analyzed species. The diameter of oocytes from adult and prepubertal animals was similar in all species, and was on average 161 ± 4 µm for oocytes with dark cytoplasm and 150 ± 18 µm for oocytes with light cytoplasm. In all species, oocytes with light cytoplasm were significantly smaller ( < 0.05) than dark ones, and their population was more diverse. Results of in vitro maturation of the domestic and wild cat's oocytes obtained from adult and prepubertal females were similar (47-52%). The cleavage rate after in vitro fertilization (IVF) was lower for prepubertal than adult domestic cats (42 vs. 51%; < 0.05%). Moreover, we observed differences in the quantity (28 vs. 39%; < 0.05) and quality of blastocysts and even greater problems with hatching blastocysts from prepubertal kittens (8 vs. 19%; < 0.001). More blastomeres were detected in blastocysts of adult cats. They also demonstrated significantly higher number of inner cell mass (ICM) ( < 0.001) and higher number of trophoblast cells (TE) ( < 0.05).

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References

Johnston L, Donoghue A, OBrien S, Wildt D . Rescue and maturation in vitro of follicular oocytes collected from nondomestic felid species. Biol Reprod. 1991; 45(6):898-906. DOI: 10.1095/biolreprod45.6.898. View

Fernandez-Gonzalez L, Hribal R, Stagegaard J, Zahmel J, Jewgenow K . Production of lion (Panthera leo) blastocysts after in vitro maturation of oocytes and intracytoplasmic sperm injection. Theriogenology. 2015; 83(6):995-9. DOI: 10.1016/j.theriogenology.2014.11.037. View

Armstrong D, Kotaras P, Earl C . Advances in production of embryos in vitro from juvenile and prepubertal oocytes from the calf and lamb. Reprod Fertil Dev. 1997; 9(3):333-9. DOI: 10.1071/r96080. View

Leoni G, Palmerini M, Satta V, Succu S, Pasciu V, Zinellu A . Differences in the Kinetic of the First Meiotic Division and in Active Mitochondrial Distribution between Prepubertal and Adult Oocytes Mirror Differences in their Developmental Competence in a Sheep Model. PLoS One. 2015; 10(4):e0124911. PMC: 4403920. DOI: 10.1371/journal.pone.0124911. View

Morton K . Developmental capabilities of embryos produced in vitro from prepubertal lamb oocytes. Reprod Domest Anim. 2008; 43 Suppl 2:137-43. DOI: 10.1111/j.1439-0531.2008.01153.x. View

Ganan N, Gonzalez R, Sestelo A, Garde J, Sanchez I, Aguilar J . Male reproductive traits, semen cryopreservation, and heterologous in vitro fertilization in the bobcat (Lynx rufus). Theriogenology. 2009; 72(3):341-52. DOI: 10.1016/j.theriogenology.2009.03.002. View

Swanson W, Maggs D, Clarke H, Newell A, Bond J, Bateman H . Assessment of viral presence in semen and reproductive function of frozen-thawed spermatozoa from Pallas' cats (Otocolobus manul) infected with feline herpesvirus. J Zoo Wildl Med. 2007; 37(3):336-46. DOI: 10.1638/05-073.1. View

Armstrong D, Holm P, Irvine B, Petersen B, Stubbings R, Mclean D . Pregnancies and live birth from in vitro fertilization of calf oocytes collected by laparoscopic follicular aspiration. Theriogenology. 1992; 38(4):667-78. DOI: 10.1016/0093-691x(92)90029-q. View

Ganan N, Gonzalez R, Garde J, Martinez F, Vargas A, Gomendio M . Assessment of semen quality, sperm cryopreservation and heterologous IVF in the critically endangered Iberian lynx (Lynx pardinus). Reprod Fertil Dev. 2009; 21(7):848-59. DOI: 10.1071/RD08226. View

10.

Ferreira E, Vireque A, Adona P, Meirelles F, Ferriani R, Navarro P . Cytoplasmic maturation of bovine oocytes: structural and biochemical modifications and acquisition of developmental competence. Theriogenology. 2009; 71(5):836-48. DOI: 10.1016/j.theriogenology.2008.10.023. View

11.

Ledda S, Bogliolo L, Calvia P, Leoni G, Naitana S . Meiotic progression and developmental competence of oocytes collected from juvenile and adult ewes. J Reprod Fertil. 1997; 109(1):73-8. DOI: 10.1530/jrf.0.1090073. View

12.

Gomez M, Pope C, Giraldo A, Lyons L, Harris R, King A . Birth of African Wildcat cloned kittens born from domestic cats. Cloning Stem Cells. 2005; 6(3):247-58. DOI: 10.1089/clo.2004.6.247. View

13.

Ptak G, Loi P, Dattena M, Tischner M, Cappai P . Offspring from one-month-old lambs: studies on the developmental capability of prepubertal oocytes. Biol Reprod. 1999; 61(6):1568-74. DOI: 10.1095/biolreprod61.6.1568. View

14.

Pope C . Aspects of in vivo oocyte production, blastocyst development, and embryo transfer in the cat. Theriogenology. 2013; 81(1):126-37. DOI: 10.1016/j.theriogenology.2013.09.006. View

15.

Johnston L, OBrien S, Wildt D . In vitro maturation and fertilization of domestic cat follicular oocytes. Gamete Res. 1989; 24(3):343-56. DOI: 10.1002/mrd.1120240310. View

16.

Morato R, Conforti V, Azevedo F, Jacomo A, Silveira L, Sana D . Comparative analyses of semen and endocrine characteristics of free-living versus captive jaguars (Panthera onca). Reproduction. 2001; 122(5):745-51. DOI: 10.1530/rep.0.1220745. View

17.

Kochan J, Nizanski W, Moreira N, Cubas Z, Nowak A, Prochowska S . ARTs in Wild Felid Conservation Programmes in Poland and in the World. J Vet Res. 2019; 63(3):457-464. PMC: 6749728. DOI: 10.2478/jvetres-2019-0043. View

18.

Roth T, Swanson W, Collins D, Burton M, Garell D, Wildt D . Snow leopard (Panthera uncia) spermatozoa are sensitive to alkaline pH, but motility in vitro is not influenced by protein or energy supplements. J Androl. 1996; 17(5):558-66. View

19.

Pope C, Johnson C, McRae M, Keller G, Dresser B . Development of embryos produced by intracytoplasmic sperm injection of cat oocytes. Anim Reprod Sci. 1998; 53(1-4):221-36. DOI: 10.1016/s0378-4320(98)00115-8. View

20.

Donoghue A, Johnston L, Seal U, Armstrong D, Tilson R, Wolf P . In vitro fertilization and embryo development in vitro and in vivo in the tiger (Panthera tigris). Biol Reprod. 1990; 43(5):733-44. DOI: 10.1095/biolreprod43.5.733. View