Virus-Like Particle Mediated CRISPR/Cas9 Delivery for Efficient and Safe Genome Editing

Overview

Journal Life (Basel)

Specialty Biology

Date 2020 Dec 29

PMID 33371215

Citations 26

Authors

Pin Lyu

Luxi Wang

Baisong Lu

Affiliations

Soon will be listed here.

Abstract

The discovery of designer nucleases has made genome editing much more efficient than before. The designer nucleases have been widely used for mechanistic studies, animal model generation and gene therapy development. However, potential off-targets and host immune responses are issues still need to be addressed for uses, especially clinical applications. Short term expression of the designer nucleases is necessary to reduce both risks. Currently, various delivery methods are being developed for transient expression of designer nucleases including Zinc Finger Nuclease (ZNF), Transcription Activator-Like Effector Nuclease (TALEN) and Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas). Recently, virus-like particles are being used for gene editing. In this review, we will talk through commonly used genome editing nucleases, discuss gene editing delivery tools and review the latest literature using virus-like particles to deliver gene editing effectors.

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