HSV-1 H129-Derived Anterograde Neural Circuit Tracers: Improvements, Production, and Applications

Overview

Journal Neurosci Bull

Specialty Neurology

Date 2020 Dec 28

PMID 33367996

Citations 13

Authors

Hong Yang

Feng Xiong

Yi-Ge Song

Hai-Fei Jiang

Hai-Bin Qin

Jing Zhou

Sha Lu

Steven F Grieco

Xiangmin Xu

Wen-Bo Zeng

Fei Zhao

Min-Hua Luo

Affiliations

Soon will be listed here.

Abstract

Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. They have been developed from herpes simplex virus 1 (HSV-1) strain H129 (H129), and have been successfully applied to map diverse neural circuits. Initially, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT, which was highly successful, yet improvements are needed. Now, by inserting another tdTomato expression cassette into the H129-dTK-tdT genome, we have created H129-dTK-T2, an updated version of H129-dTK-tdT that has improved labeling intensity. To help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.

Citing Articles

A review of HSV pathogenesis, vaccine development, and advanced applications.

Bai L, Xu J, Zeng L, Zhang L, Zhou F Mol Biomed. 2024; 5(1):35.

PMID: 39207577 PMC: 11362470. DOI: 10.1186/s43556-024-00199-7.

Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain: A Comprehensive Guide.

Li Y, Fang Y, Li K, Yang H, Duan S, Sun L Neurosci Bull. 2024; 40(9):1364-1378.

PMID: 38700806 PMC: 11365912. DOI: 10.1007/s12264-024-01196-0.

Oncolytic varicella-zoster virus engineered with ORF8 deletion and armed with drug-controllable interleukin-12.

Jiang H, Nace R, Carrasco T, Zhang L, Peng K, Russell S J Immunother Cancer. 2024; 12(3).

PMID: 38527762 PMC: 10966791. DOI: 10.1136/jitc-2023-008307.

RanBP2/Nup358 Mediates Sumoylation of STAT1 and Antagonizes Interferon-α-Mediated Antiviral Innate Immunity.

Li J, Su L, Jiang J, Wang Y, Ling Y, Qiu Y Int J Mol Sci. 2024; 25(1).

PMID: 38203469 PMC: 10778711. DOI: 10.3390/ijms25010299.

Whole-brain Optical Imaging: A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level.

Jiang T, Gong H, Yuan J Neurosci Bull. 2023; 39(12):1840-1858.

PMID: 37715920 PMC: 10661546. DOI: 10.1007/s12264-023-01112-y.

References

McGovern A, Davis-Poynter N, Farrell M, Mazzone S . Transneuronal tracing of airways-related sensory circuitry using herpes simplex virus 1, strain H129. Neuroscience. 2012; 207:148-66. DOI: 10.1016/j.neuroscience.2012.01.029. View

Wang H, Zhu Q, Ding L, Shen Y, Yang C, Xu F . Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution. Natl Sci Rev. 2021; 6(5):982-992. PMC: 8291554. DOI: 10.1093/nsr/nwz053. View

Wojaczynski G, Engel E, Steren K, Enquist L, Card J . The neuroinvasive profiles of H129 (herpes simplex virus type 1) recombinants with putative anterograde-only transneuronal spread properties. Brain Struct Funct. 2014; 220(3):1395-420. PMC: 4152387. DOI: 10.1007/s00429-014-0733-9. View

Nassi J, Cepko C, Born R, Beier K . Neuroanatomy goes viral!. Front Neuroanat. 2015; 9:80. PMC: 4486834. DOI: 10.3389/fnana.2015.00080. View

McGovern A, Davis-Poynter N, Rakoczy J, Phipps S, Simmons D, Mazzone S . Anterograde neuronal circuit tracing using a genetically modified herpes simplex virus expressing EGFP. J Neurosci Methods. 2012; 209(1):158-67. DOI: 10.1016/j.jneumeth.2012.05.035. View

Blaho J, Morton E, Yedowitz J . Herpes simplex virus: propagation, quantification, and storage. Curr Protoc Microbiol. 2008; Chapter 14:Unit 14E.1. DOI: 10.1002/9780471729259.mc14e01s00. View

Pi G, Gao D, Wu D, Wang Y, Lei H, Zeng W . Posterior basolateral amygdala to ventral hippocampal CA1 drives approach behaviour to exert an anxiolytic effect. Nat Commun. 2020; 11(1):183. PMC: 6954243. DOI: 10.1038/s41467-019-13919-3. View

Zeng W, Jiang H, Gang Y, Song Y, Shen Z, Yang H . Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129. Mol Neurodegener. 2017; 12(1):38. PMC: 5427628. DOI: 10.1186/s13024-017-0179-7. View

Zingg B, Chou X, Zhang Z, Mesik L, Liang F, Tao H . AAV-Mediated Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined Neural Pathways for Defense Behaviors. Neuron. 2016; 93(1):33-47. PMC: 5538794. DOI: 10.1016/j.neuron.2016.11.045. View

10.

Su P, Wang H, Xia J, Zhong X, Hu L, Li Y . Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer. J Chem Neuroanat. 2019; 100:101662. DOI: 10.1016/j.jchemneu.2019.101662. View

11.

Chen Y, Keiser M, Davidson B . Adeno-Associated Virus Production, Purification, and Titering. Curr Protoc Mouse Biol. 2018; 8(4):e56. DOI: 10.1002/cpmo.56. View

12.

Wickersham I, Sullivan H, Seung H . Axonal and subcellular labelling using modified rabies viral vectors. Nat Commun. 2013; 4:2332. PMC: 5939574. DOI: 10.1038/ncomms3332. View

13.

Keiser M, Chen Y, Davidson B . Techniques for Intracranial Stereotaxic Injections of Adeno-Associated Viral Vectors in Adult Mice. Curr Protoc Mouse Biol. 2018; 8(4):e57. DOI: 10.1002/cpmo.57. View

14.

Li Y, Xu J, Liu Y, Zhu J, Liu N, Zeng W . A distinct entorhinal cortex to hippocampal CA1 direct circuit for olfactory associative learning. Nat Neurosci. 2017; 20(4):559-570. DOI: 10.1038/nn.4517. View

15.

Kelly R, Strick P . Rabies as a transneuronal tracer of circuits in the central nervous system. J Neurosci Methods. 2000; 103(1):63-71. DOI: 10.1016/s0165-0270(00)00296-x. View

16.

Dum R, Levinthal D, Strick P . The spinothalamic system targets motor and sensory areas in the cerebral cortex of monkeys. J Neurosci. 2009; 29(45):14223-35. PMC: 2796243. DOI: 10.1523/JNEUROSCI.3398-09.2009. View

17.

Vaughan C, Bartness T . Anterograde transneuronal viral tract tracing reveals central sensory circuits from brown fat and sensory denervation alters its thermogenic responses. Am J Physiol Regul Integr Comp Physiol. 2012; 302(9):R1049-58. PMC: 3362143. DOI: 10.1152/ajpregu.00640.2011. View

18.

BARNETT E, Evans G, Sun N, Perlman S, Cassell M . Anterograde tracing of trigeminal afferent pathways from the murine tooth pulp to cortex using herpes simplex virus type 1. J Neurosci. 1995; 15(4):2972-84. PMC: 6577775. View

19.

Sun N, Cassell M, Perlman S . Anterograde, transneuronal transport of herpes simplex virus type 1 strain H129 in the murine visual system. J Virol. 1996; 70(8):5405-13. PMC: 190498. DOI: 10.1128/JVI.70.8.5405-5413.1996. View

20.

Garner J, LaVail J . Differential anterograde transport of HSV type 1 viral strains in the murine optic pathway. J Neurovirol. 1999; 5(2):140-50. DOI: 10.3109/13550289909021996. View