Membrane-embedded TSPO: an NMR View

Overview

Journal Eur Biophys J

Specialty Biophysics

Date 2020 Dec 23

PMID 33354729

Citations 3

Authors

Gwladys Riviere

Garima Jaipuria

Loren B Andreas

Andrei Leonov

Karin Giller

Stefan Becker

Markus Zweckstetter

Affiliations

Soon will be listed here.

Abstract

Translocator Protein (18 kDa) (TSPO) is a mitochondrial transmembrane protein commonly used as a biomarker for neuroinflammation and is also a potential therapeutic target in neurodegenerative diseases. Despite intensive research efforts, the function of TSPO is still largely enigmatic. Deciphering TSPO structure in the native lipid environment is essential to gain insight into its cellular activities and to design improved diagnostic and therapeutic ligands. Here, we discuss the influence of lipid composition on the structure of mammalian TSPO embedded into lipid bilayers on the basis of solid-state NMR experiments. We further highlight that cholesterol can influence both the tertiary and quaternary TSPO structure and also influence TSPO localization in mitochondria-associated endoplasmic reticulum membranes.

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Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment.

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Special issue: Multicomponent lipid membranes-how molecular organisation leads to function.

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