A Ubiquitin Switch Controls Autocatalytic Inactivation of the DNA-protein Crosslink Repair Protease SPRTN

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2020 Dec 21

PMID 33348378

Citations 20

Authors

Shubo Zhao

Anja Kieser

Hao-Yi Li

Hannah K Reinking

Pedro Weickert

Simon Euteneuer

Denitsa Yaneva

Aleida C Acampora

Maximilian J Gotz

Regina Feederle

Julian Stingele

Affiliations

Soon will be listed here.

Abstract

Repair of covalent DNA-protein crosslinks (DPCs) by the metalloprotease SPRTN prevents genome instability, premature aging and carcinogenesis. SPRTN is specifically activated by DNA structures containing single- and double-stranded features, but degrades the protein components of DPCs promiscuously and independent of amino acid sequence. This lack of specificity is useful to target diverse protein adducts, however, it requires tight control in return, in order to prohibit uncontrolled proteolysis of chromatin proteins. Here, we discover the components and principles of a ubiquitin switch, which negatively regulates SPRTN. We demonstrate that monoubiquitylation is induced in an E3 ligase-independent manner and, in contrast to previous assumptions, does not control chromatin access of the enzyme. Data obtained in cells and in vitro reveal that monoubiquitylation induces inactivation of the enzyme by triggering autocatalytic cleavage in trans while also priming SPRTN for proteasomal degradation in cis. Finally, we show that the deubiquitylating enzyme USP7 antagonizes this negative control of SPRTN in the presence of DPCs.

Citing Articles

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