16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis

Overview

Journal Transl Vis Sci Technol

Specialties Biomedical Engineering
Ophthalmology

Date 2020 Dec 21

PMID 33344046

Citations 11

Authors

Tobi F Somerville

Caroline E Corless

Henri Sueke

Timothy Neal

Stephen B Kaye

Affiliations

Soon will be listed here.

Abstract

Purpose: The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis.

Methods: Samples collected from 100 consecutive patients presenting to the Royal Liverpool University Hospital with bacterial keratitis were processed using CDC and 16S PCR analysis.

Results: The overall detection rate using both methods was 36%. Of these, 72.2% (26/36) were detected by PCR and 63.9% (23/36) isolated by CDC ( = 0.62). Using a combination of both PCR and CDC increased the detection rate for pathogenic bacteria by 13% compared to using CDC alone ( = 0.04). In CDC negative samples, 16S PCR identified more pathogens than CDC in 16S PCR negative samples. Neither order of sample collection nor prior antimicrobial use affected the detection rate.

Conclusions: 16S rRNA gene PCR performed in addition to CDC on corneal samples from patients with clinically suspected bacterial keratitis led to additional pathogen detection.

Translational Relevance: 16S rRNA gene PCR should be developed to become an additional part of clinical service for patients with bacterial keratitis rather than used in isolation.

Citing Articles

Clinical features, risk factors and outcomes of contact lens-related bacterial keratitis in Nottingham, UK: a 7-year study.

Suresh L, Hammoudeh Y, Ho C, Ong Z, Cairns J, Gopal B Eye (Lond). 2024; 38(18):3459-3466.

PMID: 39261654 PMC: 11621543. DOI: 10.1038/s41433-024-03323-7.

Microbiological culture versus 16S/18S rRNA gene PCR-sanger sequencing for infectious keratitis: a three-arm, diagnostic cross-sectional study.

Hammoudeh Y, Suresh L, Ong Z, Lister M, Mohammed I, Thomas D Front Med (Lausanne). 2024; 11:1393832.

PMID: 39206175 PMC: 11352289. DOI: 10.3389/fmed.2024.1393832.

Microbial keratitis in Southern Malawi: a microbiological pilot study.

Somerville T, Mdala S, Zungu T, Gandiwa M, Herbert R, Everett D BMJ Open Ophthalmol. 2024; 9(1).

PMID: 38653537 PMC: 11043707. DOI: 10.1136/bmjophth-2024-001682.

Diagnosis of Keratitis: Past, Present and Future.

Azzopardi M, Chong Y, Ng B, Recchioni A, Logeswaran A, Ting D Diagnostics (Basel). 2023; 13(16).

PMID: 37627913 PMC: 10453105. DOI: 10.3390/diagnostics13162655.

Usefulness of smear microscopy for therapeutic decision-making in patients with infectious keratitis.

Mitoma K, Chikama T, Toda R, Yuasa Y, Kiuchi Y Jpn J Ophthalmol. 2023; 67(5):570-577.

PMID: 37439922 DOI: 10.1007/s10384-023-01011-9.

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