Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2020 Dec 19

PMID 33340355

Authors

Thomas Richardson

Shibin Mathew

Connor Wiegand

Kevin Pietz

Joseph Candiello

K Ravikumar

Ipsita Banerjee

Affiliations

Soon will be listed here.

Abstract

Biophysical cues synergize with biochemical cues to drive differentiation of pluripotent stem cells through specific phenotypic trajectory. Tools to manipulate the cell biophysical environment and identify the influence of specific environment perturbation in the presence of combinatorial inputs will be critical to control the development trajectory. Here we describe the procedure to perturb biophysical environment of pluripotent stem cells while maintaining them in 3D culture configuration. We also discuss a high-throughput platform for combinatorial perturbation of the cell microenvironment, and detail a statistical procedure to extract dominant environmental influences.

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