Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2020 Dec 18

PMID 33338433

Citations 10

Authors

Chi-Hun Park

Young-Hee Jeoung

Kyung-Jun Uh

Ki-Eun Park

Jessica Bridge

Anne Powell

Jie Li

Laramie Pence

Luhui Zhang

Tianbin Liu

Hai-Xi Sun

Ying Gu

Yue Shen

Jun Wu

Juan-Carlos Izpisua Belmonte

Bhanu P Telugu

Affiliations

Soon will be listed here.

Abstract

Most of our current knowledge regarding early lineage specification and embryo-derived stem cells comes from studies in rodent models. However, key gaps remain in our understanding of these developmental processes from nonrodent species. Here, we report the detailed characterization of pig extraembryonic endoderm (pXEN) cells, which can be reliably and reproducibly generated from primitive endoderm (PrE) of blastocyst. Highly expandable pXEN cells express canonical PrE markers and transcriptionally resemble rodent XENs. The pXEN cells contribute both to extraembryonic tissues including visceral yolk sac as well as embryonic gut when injected into host blastocysts, and generate live offspring when used as a nuclear donor in somatic cell nuclear transfer (SCNT). The pXEN cell lines provide a novel model for studying lineage segregation, as well as a source for genome editing in livestock.

Citing Articles

Defining core signaling pathways for supporting in vitro maintenance of pig extraembryonic endoderm (XEN) cells.

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Establishment of bovine extraembryonic endoderm cells.

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