Identification and Characterization of a Novel Multi-stress Responsive Gene in Arabidopsis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Dec 17

PMID 33332435

Citations 3

Authors

Faiza Tawab

Iqbal Munir

Zeeshan Nasim

Mohammad Sayyar Khan

Saleha Tawab

Adnan Nasim

Aqib Iqbal

Mian Afaq Ahmad

Waqar Ali

Raheel Munir

Maria Munir

Noreen Asim

Affiliations

Soon will be listed here.

Abstract

Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.

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