The CSF-Contacting Nucleus Receives Anatomical Inputs From the Cerebral Cortex: A Combination of Retrograde Tracing and 3D Reconstruction Study in Rat

Overview

Journal Front Neuroanat

Date 2020 Dec 17

PMID 33328908

Citations 1

Authors

Si-Yuan Song

Xiao-Meng Zhai

Jia-Hao Dai

Lei-Lei Lu

Cheng-Jing Shan

Jia Hong

Jun-Li Cao

Li-Cai Zhang

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate the direct monosynaptic projections from cortical functional regions to the cerebrospinal fluid (CSF)-contacting nucleus for understanding the functions of the CSF-contacting nucleus.

Methods: The Sprague-Dawley rats received cholera toxin B subunit (CB) injections into the CSF-contacting nucleus. After 7-10 days of survival time, the rats were perfused, and the whole brain and spinal cord were sliced under a freezing microtome at 40 μm. All sections were treated with the CB immunofluorescence reaction. The retrogradely labeled neurons in different cortical areas were revealed under a confocal microscope. The distribution features were further illustrated under 3D reconstruction.

Results: The retrogradely labeled neurons were identified in the olfactory, orbital, cingulate, insula, retrosplenial, somatosensory, motor, visual, auditory, association, rhinal, and parietal cortical areas. A total of 12 functional areas and 34 functional subregions showed projections to the CSF-contacting nucleus in different cell intensities.

Conclusion: According to the connectivity patterns, we conclude that the CSF-contacting nucleus participates in cognition, emotion, pain, visceral activity, etc. The present study firstly reveals the cerebral cortex→CSF-contacting nucleus connections, which implies the multiple functions of this special nucleus in neural and body fluid regulations.

Citing Articles

Genetic Architecture and Functional Implications of the CSF-Contacting Nucleus.

Song S, Yuan Y, Xu L, Jiang J, Li Y, Yan Y Neurosci Bull. 2023; 39(11):1638-1654.

PMID: 37405574 PMC: 10602992. DOI: 10.1007/s12264-023-01084-z.

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