Injectable Magnesium-Zinc Alloy Containing Hydrogel Complex for Bone Regeneration

Overview

Journal Front Bioeng Biotechnol

Date 2020 Dec 16

PMID 33324628

Citations 7

Authors

Wei-Hua Wang

Fei Wang

Hai-Feng Zhao

Ke Yan

Cui-Ling Huang

Yin Yin

Qiang Huang

Zao-Zao Chen

Wen-Yu Zhu

Affiliations

Soon will be listed here.

Abstract

Gelatin methacryloyl (GelMA) has been widely used in bone engineering. It can also be filled into the calvarial defects with irregular shape. However, lack of osteoinductive capacity limits its potential as a candidate repair material for calvarial defects. In this study, we developed an injectable magnesium-zinc alloy containing hydrogel complex (Mg-IHC), in which the alloy was fabricated in an atomization process and had small sphere, regular shape, and good fluidity. Mg-IHC can be injected and plastically shaped. After cross-linking, it contents the elastic modulus similar to GelMA, and has inner holes suitable for nutrient transportation. Furthermore, Mg-IHC showed promising biocompatibility according to our evaluations of its cell adhesion, growth status, and proliferating activity. The results of alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, and real-time polymerase chain reaction (PCR) further indicated that Mg-IHC could significantly promote the osteogenic differentiation of MC3T3-E1 cells and upregulate the genetic expression of collagen I (COL-I), osteocalcin (OCN), and runt-related transcription factor 2 (RUNX2). Finally, after applied to a mouse model of critical-sized calvarial defect, Mg-IHC remarkably enhanced bone formation at the defect site. All of these results suggest that Mg-IHC can promote bone regeneration and can be potentially considered as a candidate for calvarial defect repairing.

Citing Articles

Bone Regeneration Induced by Patient-Adapted Mg Alloy-Based Scaffolds for Bone Defects: Present and Future Perspectives.

Manescu Paltanea V, Antoniac I, Antoniac A, Laptoiu D, Paltanea G, Ciocoiu R Biomimetics (Basel). 2023; 8(8).

PMID: 38132557 PMC: 10742271. DOI: 10.3390/biomimetics8080618.

Development of a composite hydrogel incorporating anti-inflammatory and osteoinductive nanoparticles for effective bone regeneration.

Byun H, Jang G, Jeong H, Lee J, Huh S, Lee S Biomater Res. 2023; 27(1):132.

PMID: 38087321 PMC: 10717596. DOI: 10.1186/s40824-023-00473-9.

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment.

Lv B, Lu L, Hu L, Cheng P, Hu Y, Xie X Theranostics. 2023; 13(6):2015-2039.

PMID: 37064871 PMC: 10091878. DOI: 10.7150/thno.80615.

Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface.

Wang T, Xu W, Zhao X, Bai B, Hua Y, Tang J Mater Today Bio. 2022; 14:100234.

PMID: 35308043 PMC: 8924418. DOI: 10.1016/j.mtbio.2022.100234.

Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration.

Li J, Wang W, Li M, Song P, Lei H, Gui X Front Bioeng Biotechnol. 2021; 9:770049.

PMID: 34926420 PMC: 8675867. DOI: 10.3389/fbioe.2021.770049.

References

Wohlrab S, Muller S, Schmidt A, Neubauer S, Kessler H, Leal-Egana A . Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins. Biomaterials. 2012; 33(28):6650-9. DOI: 10.1016/j.biomaterials.2012.05.069. View

Vega S, Kwon M, Burdick J . Recent advances in hydrogels for cartilage tissue engineering. Eur Cell Mater. 2017; 33:59-75. PMC: 5748291. DOI: 10.22203/eCM.v033a05. View

Yue K, Trujillo-de Santiago G, Alvarez M, Tamayol A, Annabi N, Khademhosseini A . Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials. 2015; 73:254-71. PMC: 4610009. DOI: 10.1016/j.biomaterials.2015.08.045. View

Yan Y, Wei Y, Yang R, Xia L, Zhao C, Gao B . Enhanced osteogenic differentiation of bone mesenchymal stem cells on magnesium-incorporated titania nanotube arrays. Colloids Surf B Biointerfaces. 2019; 179:309-316. DOI: 10.1016/j.colsurfb.2019.04.013. View

Ma J, Wang J, Ai X, Zhang S . Biomimetic self-assembly of apatite hybrid materials: from a single molecular template to bi-/multi-molecular templates. Biotechnol Adv. 2013; 32(4):744-60. DOI: 10.1016/j.biotechadv.2013.10.014. View

Qiao Y, Liu X, Zhou X, Zhang H, Zhang W, Xiao W . Gelatin Templated Polypeptide Co-Cross-Linked Hydrogel for Bone Regeneration. Adv Healthc Mater. 2019; 9(1):e1901239. DOI: 10.1002/adhm.201901239. View

Chen S, Shi Y, Zhang X, Ma J . Biomimetic synthesis of Mg-substituted hydroxyapatite nanocomposites and three-dimensional printing of composite scaffolds for bone regeneration. J Biomed Mater Res A. 2019; 107(11):2512-2521. DOI: 10.1002/jbm.a.36757. View

Wolf F, Cittadini A . Chemistry and biochemistry of magnesium. Mol Aspects Med. 2003; 24(1-3):3-9. DOI: 10.1016/s0098-2997(02)00087-0. View

Zhang K, Lin S, Feng Q, Dong C, Yang Y, Li G . Nanocomposite hydrogels stabilized by self-assembled multivalent bisphosphonate-magnesium nanoparticles mediate sustained release of magnesium ion and promote in-situ bone regeneration. Acta Biomater. 2017; 64:389-400. DOI: 10.1016/j.actbio.2017.09.039. View

10.

Liu Y, Chan-Park M . A biomimetic hydrogel based on methacrylated dextran-graft-lysine and gelatin for 3D smooth muscle cell culture. Biomaterials. 2009; 31(6):1158-70. DOI: 10.1016/j.biomaterials.2009.10.040. View

11.

Zberg B, Uggowitzer P, Loffler J . MgZnCa glasses without clinically observable hydrogen evolution for biodegradable implants. Nat Mater. 2009; 8(11):887-91. DOI: 10.1038/nmat2542. View

12.

Yu X, Shou W, Mahajan B, Huang X, Pan H . Materials, Processes, and Facile Manufacturing for Bioresorbable Electronics: A Review. Adv Mater. 2018; 30(28):e1707624. DOI: 10.1002/adma.201707624. View

13.

Kwon S, Lee S, Sivashanmugam A, Kwon J, Kim S, Noh M . Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects. Polymers (Basel). 2019; 10(8). PMC: 6403913. DOI: 10.3390/polym10080914. View

14.

Nabiyouni M, Ren Y, Bhaduri S . Magnesium substitution in the structure of orthopedic nanoparticles: A comparison between amorphous magnesium phosphates, calcium magnesium phosphates, and hydroxyapatites. Mater Sci Eng C Mater Biol Appl. 2015; 52:11-7. DOI: 10.1016/j.msec.2015.03.032. View

15.

Wang G, Li J, Zhang W, Xu L, Pan H, Wen J . Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function. Int J Nanomedicine. 2014; 9:2387-98. PMC: 4051717. DOI: 10.2147/IJN.S58357. View

16.

Naujokat H, Seitz J, Acil Y, Damm T, Moller I, Gulses A . Osteosynthesis of a cranio-osteoplasty with a biodegradable magnesium plate system in miniature pigs. Acta Biomater. 2017; 62:434-445. DOI: 10.1016/j.actbio.2017.08.031. View

17.

Karageorgiou V, Kaplan D . Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005; 26(27):5474-91. DOI: 10.1016/j.biomaterials.2005.02.002. View

18.

Zhu W, Wu J, Zhao H, Wang W, Lu L, Yan K . Establishment and Characteristic Analysis of a Dog Model for Autologous Homologous Cranioplasty. Biomed Res Int. 2020; 2020:5324719. PMC: 7273410. DOI: 10.1155/2020/5324719. View

19.

Lucateli R, Marciano M, Ferreira S, Junior I, Camilleri J, Mariano R . Doxycycline and Autogenous Bone in Repair of Critical-Size Defects. Implant Dent. 2018; 27(4):461-466. DOI: 10.1097/ID.0000000000000783. View

20.

Borrelli M, Hu M, Hong W, Oliver J, Duscher D, Longaker M . Macrophage Transplantation Fails to Improve Repair of Critical-Sized Calvarial Defects. J Craniofac Surg. 2019; 30(8):2640-2645. PMC: 7089774. DOI: 10.1097/SCS.0000000000005797. View