Unconventional Secretion of Nigerolysins A from Species

Overview

Journal Microorganisms

Specialty Microbiology

Date 2020 Dec 16

PMID 33322461

Citations 5

Authors

Nada Krasevec

Marusa Novak

Simona Barat

Matej Skocaj

Kristina Sepcic

Gregor Anderluh

Affiliations

Soon will be listed here.

Abstract

Aegerolysins are small lipid-binding proteins particularly abundant in fungi. Aegerolysins from oyster mushrooms interact with an insect-specific membrane lipid and, together with MACPF proteins produced by the same organism, form pesticidal pore-forming complexes. The specific interaction with the same membrane lipid was recently demonstrated for nigerolysin A2 (NigA2), an aegerolysin from . In species, the aegerolysins were frequently found as secreted proteins, indicating their function in fungal defense. Using immunocytochemistry and live-cell imaging we investigated the subcellular localization of the nigerolysins A in , while their secretion was addressed by secretion prediction and Western blotting. We show that both nigerolysins A are leaderless proteins that reach the cell exterior by an unconventional protein secretion. NigA proteins are evenly distributed in the cytoplasm of fungal hyphae. A detailed bioinformatics analysis of aegerolysins suggests that the same function occurs only in a limited number of aegerolysins. From alignment, analysis of chromosomal loci, orthology, synteny, and phylogeny it follows that the same or a similar function described for pairs of pesticidal proteins of sp. can be expected in species of the subgenus , section , series , and some other species with adjacent pairs of putative pesticidal proteins.

Citing Articles

New Insights into Interactions between Mushroom Aegerolysins and Membrane Lipids.

Poposek L, Krasevec N, Bajc G, Glavac U, Hrovatin M, Perko Z Toxins (Basel). 2024; 16(3).

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A bird's-eye view of the endoplasmic reticulum in filamentous fungi.

Martinez-Andrade J, Roberson R, Riquelme M Microbiol Mol Biol Rev. 2024; 88(1):e0002723.

PMID: 38372526 PMC: 10966943. DOI: 10.1128/mmbr.00027-23.

Towards Understanding the Function of Aegerolysins.

Krasevec N, Skocaj M Toxins (Basel). 2022; 14(9).

PMID: 36136567 PMC: 9505663. DOI: 10.3390/toxins14090629.

Towards a Fungal Science That Is Independent of Researchers' Gender.

Krasevec N J Fungi (Basel). 2022; 8(7).

PMID: 35887432 PMC: 9321353. DOI: 10.3390/jof8070675.

Lipid-Binding Aegerolysin from Biocontrol Fungus .

Krasevec N, Panevska A, Lemez S, Razinger J, Sepcic K, Anderluh G Toxins (Basel). 2021; 13(11).

PMID: 34822604 PMC: 8624791. DOI: 10.3390/toxins13110820.

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