Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2020 Dec 16

PMID 33322240

Citations 11

Authors

Nathalie Delaunay

Audrey Combes

Valerie Pichon

Affiliations

Soon will be listed here.

Abstract

The evolution of instrumentation in terms of separation and detection allowed a real improvement of the sensitivity and analysis time. However, the analysis of ultra-traces of toxins in complex samples requires often a step of purification and even preconcentration before their chromatographic analysis. Therefore, immunoaffinity sorbents based on specific antibodies thus providing a molecular recognition mechanism appear as powerful tools for the selective extraction of a target molecule and its structural analogs to obtain more reliable and sensitive quantitative analysis in environmental, food or biological matrices. This review focuses on immunosorbents that have proven their efficiency in selectively extracting various types of toxins of various sizes (from small mycotoxins to large proteins) and physicochemical properties. Immunosorbents are now commercially available, and their use has been validated for numerous applications. The wide variety of samples to be analyzed, as well as extraction conditions and their impact on extraction yields, is discussed. In addition, their potential for purification and thus suppression of matrix effects, responsible for quantification problems especially in mass spectrometry, is presented. Due to their similar properties, molecularly imprinted polymers and aptamer-based sorbents that appear to be an interesting alternative to antibodies are also briefly addressed by comparing their potential with that of immunosorbents.

Citing Articles

Occurrence of mycotoxins in milk thistle: to be included in legislation or not?.

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Mycotoxins in Food: Cancer Risks and Strategies for Control.

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[Recent advances of molecular imprinting technology for the separation and recognition of complex biological sample systems].

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PMID: 38845512 PMC: 11165394. DOI: 10.3724/SP.J.1123.2024.01011.

Comprehensive Insights into Ochratoxin A: Occurrence, Analysis, and Control Strategies.

Ben Miri Y, Benabdallah A, Chentir I, Djenane D, Luvisi A, De Bellis L Foods. 2024; 13(8).

PMID: 38672856 PMC: 11049263. DOI: 10.3390/foods13081184.

Dispersive solid phase extraction using a hydrophilic molecularly imprinted polymer for the selective extraction of patulin in apple juice samples.

Cavaliere C, Cerrato A, Lagana A, Montone C, Piovesana S, Taglioni E Mikrochim Acta. 2023; 190(12):485.

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