Toward a Better Definition of Hematopoietic Progenitors Suitable for B Cell Differentiation

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Dec 15

PMID 33320872

Citations 2

Authors

Florian Dubois

Anne Gaignerie

Lea Flippe

Jean-Marie Heslan

Laurent Tesson

Melanie Chesneau

Fabienne Haspot

Sophie Conchon

Laurent David

Sophie Brouard

Affiliations

Soon will be listed here.

Abstract

The success of inducing human pluripotent stem cells (hIPSC) offers new opportunities for cell-based therapy. Since B cells exert roles as effector and as regulator of immune responses in different clinical settings, we were interested in generating B cells from hIPSC. We differentiated human embryonic stem cells (hESC) and hIPSC into B cells onto OP9 and MS-5 stromal cells successively. We overcame issues in generating CD34+CD43+ hematopoietic progenitors with appropriate cytokine conditions and emphasized the difficulties to generate proper hematopoietic progenitors. We highlight CD31intCD45int phenotype as a possible marker of hematopoietic progenitors suitable for B cell differentiation. Defining precisely proper lymphoid progenitors will improve the study of their lineage commitment and the signals needed during the in vitro process.

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