Extracts of Medicinal Plants with Natural Deep Eutectic Solvents: Enhanced Antimicrobial Activity and Low Genotoxicity

Overview

Journal BMC Chem

Publisher Springer Nature

Specialty Chemistry

Date 2020 Dec 14

PMID 33308280

Citations 14

Authors

Tsvetinka Grozdanova

Boryana Trusheva

Kalina Alipieva

Milena Popova

Lyudmila Dimitrova

Hristo Najdenski

Maya M Zaharieva

Yana Ilieva

Bela Vasileva

George Miloshev

Milena Georgieva

Vassya Bankova

Affiliations

Soon will be listed here.

Abstract

Natural deep eutectic solvents (NADES) are a new alternative to toxic organic solvents. Their constituents are primary metabolites, non-toxic, biocompatible and sustainable. In this study four selected NADES were applied for the extraction of two medicinal plants: Sideritis scardica, and Plantago major as an alternative to water-alcohol mixtures, and the antimicrobial and genotoxic potential of the extracts were studied. The extraction efficiency was evaluated by measuring the extracted total phenolics, and total flavonoids. Best extraction results for total phenolics for the studied plants were obtained with choline chloride-glucose 5:2 plus 30% water; but surprisingly these extracts were inactive against all tested microorganisms. Extracts with citric acid-1,2-propanediol 1:4 and choline chloride-glycerol 1:2 showed good activity against S. pyogenes, E. coli, S. aureus, and C. albicans. Low genotoxicity and cytotoxicity were observed for all four NADES and the extracts with antimicrobial activity. Our results confirm the potential of NADESs for extraction of bioactive constituents of medicinal plants and further suggest that NADES can improve the effects of bioactive extracts. Further studies are needed to clarify the influence of the studied NADES on the bioactivity of dissolved substances, and the possibility to use such extracts in the pharmaceutical and food industry.

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References

Dai Y, Witkamp G, Verpoorte R, Choi Y . Tailoring properties of natural deep eutectic solvents with water to facilitate their applications. Food Chem. 2015; 187:14-9. DOI: 10.1016/j.foodchem.2015.03.123. View

Wikene K, Rukke H, Bruzell E, Tonnesen H . Investigation of the antimicrobial effect of natural deep eutectic solvents (NADES) as solvents in antimicrobial photodynamic therapy. J Photochem Photobiol B. 2017; 171:27-33. DOI: 10.1016/j.jphotobiol.2017.04.030. View

Eliaz I, Weil E, Schwarzbach J, Wilk B . Modified Citrus Pectin / Alginate Dietary Supplement Increased Fecal Excretion of Uranium: A Family. Altern Ther Health Med. 2019; 25(4):20-24. View

Samuelsen A . The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J Ethnopharmacol. 2000; 71(1-2):1-21. PMC: 7142308. DOI: 10.1016/s0378-8741(00)00212-9. View

Dimitrova L, Zaharieva M, Popova M, Kostadinova N, Tsvetkova I, Bankova V . Antimicrobial and antioxidant potential of different solvent extracts of the medicinal plant Geum urbanum L. Chem Cent J. 2017; 11(1):113. PMC: 5676587. DOI: 10.1186/s13065-017-0343-8. View

Staneva D, Peycheva E, Georgieva M, Efremov T, Miloshev G . Application of comet assay for the assessment of DNA damage caused by chemical genotoxins in the dairy yeast Kluyveromyces lactis. Antonie Van Leeuwenhoek. 2012; 103(1):143-52. DOI: 10.1007/s10482-012-9793-0. View

Collins A . The comet assay: a heavenly method!. Mutagenesis. 2014; 30(1):1-4. DOI: 10.1093/mutage/geu079. View

Bilal M, Iqbal H . An insight into toxicity and human-health-related adverse consequences of cosmeceuticals - A review. Sci Total Environ. 2019; 670:555-568. DOI: 10.1016/j.scitotenv.2019.03.261. View

Tice R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H . Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3):206-21. DOI: 10.1002/(sici)1098-2280(2000)35:3<206::aid-em8>3.0.co;2-j. View

10.

Todorova M, Trendafilova A . Sideritis scardica Griseb., an endemic species of Balkan peninsula: traditional uses, cultivation, chemical composition, biological activity. J Ethnopharmacol. 2014; 152(2):256-65. DOI: 10.1016/j.jep.2014.01.022. View

11.

Ruesgas-Ramon M, Figueroa-Espinoza M, Durand E . Application of Deep Eutectic Solvents (DES) for Phenolic Compounds Extraction: Overview, Challenges, and Opportunities. J Agric Food Chem. 2017; 65(18):3591-3601. DOI: 10.1021/acs.jafc.7b01054. View

12.

Gutierrez M, Ferrer M, Mateo C, Del Monte F . Freeze-drying of aqueous solutions of deep eutectic solvents: a suitable approach to deep eutectic suspensions of self-assembled structures. Langmuir. 2009; 25(10):5509-15. DOI: 10.1021/la900552b. View

13.

Dai Y, Van Spronsen J, Witkamp G, Verpoorte R, Choi Y . Natural deep eutectic solvents as new potential media for green technology. Anal Chim Acta. 2013; 766:61-8. DOI: 10.1016/j.aca.2012.12.019. View

14.

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

15.

Fairbairn D, Olive P, ONeill K . The comet assay: a comprehensive review. Mutat Res. 1995; 339(1):37-59. DOI: 10.1016/0165-1110(94)00013-3. View

16.

Radosevic K, canak I, Panic M, Markov K, Cvjetko Bubalo M, Frece J . Antimicrobial, cytotoxic and antioxidative evaluation of natural deep eutectic solvents. Environ Sci Pollut Res Int. 2018; 25(14):14188-14196. DOI: 10.1007/s11356-018-1669-z. View